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020 _a9781071617793
_9978-1-0716-1779-3
024 7 _a10.1007/978-1-0716-1779-3
_2doi
050 4 _aQR
072 7 _aPSG
_2bicssc
072 7 _aSCI045000
_2bisacsh
072 7 _aPSG
_2thema
082 0 4 _a579
_223
245 1 0 _aMycobacterium ulcerans
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Gerd Pluschke, Katharina Röltgen.
250 _a1st ed. 2022.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2022.
300 _aX, 232 p. 33 illus., 25 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2387
505 0 _aOverview: Mycobacterium ulcerans Disease (Buruli ulcer) -- Immunohistochemistry: A Method to Analyze M.ulcerans Infected Skin Tissue -- Primary Isolation of Mycobacterium ulcerans -- Investigation of Mycobacterium ulcerans Glycan Interactions Using Glycan Array and Surface Plasmon Resonance -- Purification and Characterization of Extracellular Vesicles from Mycobacterium ulcerans Culture -- DNA Extraction from Clinical Specimens for the Direct Detection of Mycobacterium ulcerans by Real-Time PCR -- Extraction of DNA from Environmental Samples for the Real-Time PCR Detection of Mycobacterium ulcerans -- Detection of Mycobacterium ulcerans DNA using Real-Time PCR -- Determining Viability of M. ulcerans by 16S rRNA RT Reverse Transcriptase Real-Time PCR -- Methods and Approaches for Buruli ulcer Surveillance in Africa: Lessons Learnt and Future Directions -- Overview: Mycolactone, The Macrolide Toxin of Mycobacterium ulcerans -- Conditionsfor Handling and Optimal Storage of Mycolactone -- Mycolactone Purification from M. ulcerans Cultures and HPLC-Based Approaches for Mycolactone Quantification in Biological Samples -- Detection of Mycolactone by Thin Layer Chromatography -- Competitive ELISA for the Detection and Quantification of Mycobacterium ulcerans Mycolactone -- Biochemical and Biological Assays of Mycolactone-Mediated Inhibition of Sec61 -- Overview: Development of Drugs against Mycobacterium ulcerans -- In Vitro Assessment of Drug Activity against Mycobacterium ulcerans -- Drug Efficacy Testing in the Mouse Footpad Model of Buruli ulcer -- Turbidity-Based MIC Assay and Characterization of Spontaneous Drug Resistant Mutants in Mycobacterium ulcerans -- Determination of Bioenergetic Parameters in Mycobacterium ulcerans.
520 _aThis volume explores the latest techniques used to study Mycobacterium ulcerans, and more specifically M. ulcerans disease (Buruli ulcer). The chapters in this book are organized into three parts and cover methods for the detection of M. ulcerans and the analysis of host-pathogen interaction; the quantification and characterization of mycolactone, the macrolide toxin of M. ulcerans; and drug development against M. ulcerans. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mycobacterium ulcerans: Methods and Protocols is a valuable resource that helps scientists advance their research on Buruli ulcer, which is still an under-researched field in infection biology.
650 0 _aMicrobiology.
650 0 _aImmunology.
650 0 _aPathology.
650 1 4 _aMicrobiology.
650 2 4 _aImmunology.
650 2 4 _aPathology.
700 1 _aPluschke, Gerd.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aRöltgen, Katharina.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071617786
776 0 8 _iPrinted edition:
_z9781071617809
776 0 8 _iPrinted edition:
_z9781071617816
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2387
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-1779-3
912 _aZDB-2-PRO
999 _c5910
_d5910