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020 _a9781071616772
_9978-1-0716-1677-2
024 7 _a10.1007/978-1-0716-1677-2
_2doi
050 4 _aQK731-745
050 4 _aQH514
072 7 _aPST
_2bicssc
072 7 _aSCI011000
_2bisacsh
072 7 _aPSTB
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082 0 4 _a571.82
_223
245 1 0 _aPlant Gravitropism
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Elison B. Blancaflor.
250 _a2nd ed. 2022.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2022.
300 _aXII, 323 p. 88 illus., 83 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2368
505 0 _aPlant Gravitropism: From Mechanistic Insights into Plant Function on Earth to Plants Colonizing Other Worlds -- Evaluation of Gravitropism in Non-Seed Plants -- Spore Preparation and Protoplast Isolation to Study Gravity Perception and Response in Ceratopteris richardii -- A Multiplexed, Time-Resolved Assay of Root Gravitropic Bending on Agar Plates -- Methods to Quantify Cell Division and Hormone Gradients During Root Tropisms -- Using the Automated Botanical Contact Device (ABCD) to Deliver Reproducible, Intermittent Touch Stimulation to Plants -- High-Resolution Kinematic Analysis of Root Gravitropic Bending Using RootPlot -- Staging of Emerged Lateral Roots in Arabidopsis thaliana -- Methods for a Quantitative Comparison of Gravitropism and Posture Control Over a Wide Range of Herbaceous and Woody Species -- The Analysis of Gravitropic Setpoint Angle Control in Plants -- A Flat Embedding Method to Orient Gravistimulated Root Samples for Sectioning -- Conducting Plant Experiments in Space and on the Moon -- Plant Proteomic Data Acquisition and Data Analyses: Lessons from Spaceflight -- Blueprints for Constructing Microgravity Analogs -- Arabidopsis Growth and Dissection on Polyethersulfone (PES) Membranes for Gravitropic Studies -- Use of Reduced Gravity Simulators for Plant Biological Studies -- Understanding the Mechanisms of Gravity Resistance in Plants -- NASA’s Ground-Based Microgravity Simulation Facility -- Evaluating the Effects of the Circadian Clock and Time of Day on Plant Gravitropic Responses.
520 _aThis updated and expanded edition explores key methodologies to study the fascinating phenomenon of how plants readjust their growth toward gravity. In addition to the protocols delivering broad applications for gaining insight into other plant physiological processes, this new volume also focuses on techniques involving plants in space or the use of microgravity analogs to study plant biological phenomenon. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Gravitropism: Methods and Protocols, Second Edition serves as an ideal guide for researchers studying the cellular, molecular, and biochemical networks that plants use to translate environmental stimuli into a growth response.
650 0 _aTropisms.
650 0 _aGrowth (Plants).
650 0 _aBotany.
650 1 4 _aPlant Tropism.
650 2 4 _aPlant Science.
700 1 _aBlancaflor, Elison B.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071616765
776 0 8 _iPrinted edition:
_z9781071616789
776 0 8 _iPrinted edition:
_z9781071616796
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2368
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-1677-2
912 _aZDB-2-PRO
999 _c5909
_d5909