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020 _a9781071614334
_9978-1-0716-1433-4
024 7 _a10.1007/978-1-0716-1433-4
_2doi
050 4 _aQH573-671
072 7 _aPSF
_2bicssc
072 7 _aSCI017000
_2bisacsh
072 7 _aPSF
_2thema
082 0 4 _a571.6
_223
245 1 0 _aMitochondrial Regulation
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Carlos M. Palmeira, Anabela P. Rolo.
250 _a2nd ed. 2021.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2021.
300 _aXII, 318 p. 64 illus., 47 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2310
505 0 _aIsolation of Mitochondria from Liver and Extraction of Total RNA and Protein: Analyses of miRNA and Protein Expression -- Determination of Oxidative Phosphorylation Complexes Activities -- BN-PAGE-Based Approach to Study Thyroid Hormones and Mitochondrial Function -- Monitoring Mitochondrial Function in Mouse Embryonic Stem Cells (mESCs) -- Mitochondrial Functional Assessment in Mammalian Gametes Using Fluorescent Probes -- Update on the Histoenzymatic Methods for Visualization of the Activity of Individual Mitochondrial Respiratory Chain Complexes in the Human Frozen Sections -- An Update on Isolation of Functional Mitochondria from Cell for Bioenergetics Studies -- PCR-Based Determination of Mitochondrial DNA Copy Number in Multiple Species -- Methods to Monitor Mitophagy and Mitochondrial Quality: Implications in Cancer, Neurodegeneration, and Cardiovascular Diseases -- Analysis of Pro-Apoptotic Protein Trafficking to and from Mitochondria -- Exploring Liver Mitochondrial Function by 13C-Stable Isotope Breath Tests: Implications in Clinical Biochemistry -- Protocols for Mitochondria as the Target of Pharmacological Therapy in the Context of Nonalcoholic Fatty Liver Disease (NAFLD) -- The Use of Reactive Oxygen Species Production by Succinate-Driven Reverse Electron Flow as an Index of Complex 1 Activity in Isolated Brown Adipose Tissue Mitochondria -- Mitochondrial Regulation Assessment by 13C-NMR Isotopomer Analysis -- Assays for Determination of Cellular and Mitochondrial NAD+ and NADH Content -- Following the Dynamism of the Mitochondrial Network in T Cells -- Measuring PGC-1α and Its Acetylation Status in Mouse Primary Myotubes.
520 _aThis fully updated edition explores the different pathways that converge into the regulation of mitochondrial function. The book integrates mitochondria with other cellular components, discussing the dynamic properties of mitochondria with an emphasis on how these processes respond to signaling events and how they affect cellular metabolism. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Mitochondrial Regulation: Methods and Protocols, Second Edition is an ideal guide for advanced undergraduates, graduates, postgraduates, and beginning researchers in the areas of molecular and cellular biology, biochemistry, and bioenergetics.
650 0 _aCytology.
650 0 _aBiophysics.
650 1 4 _aCell Biology.
650 2 4 _aBiophysics.
700 1 _aPalmeira, Carlos M.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aRolo, Anabela P.
_eeditor.
_0(orcid)0000-0003-3535-9630
_1https://orcid.org/0000-0003-3535-9630
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071614327
776 0 8 _iPrinted edition:
_z9781071614341
776 0 8 _iPrinted edition:
_z9781071614358
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2310
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-1433-4
912 _aZDB-2-PRO
999 _c5905
_d5905