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020 _a9781071630600
_9978-1-0716-3060-0
024 7 _a10.1007/978-1-0716-3060-0
_2doi
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_2bicssc
072 7 _aSCI045000
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072 7 _aPSG
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082 0 4 _a579.3
_223
245 1 0 _aBacterial and Archaeal Motility
_h[electronic resource] /
_cedited by Tohru Minamino, Makoto Miyata, Keiichi Namba.
250 _a1st ed. 2023.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2023.
300 _aXVI, 402 p. 105 illus., 81 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2646
505 0 _aPurification of the Transmembrane Polypeptide Channel Complex of the Salmonella Flagellar Type III Secretion System -- In Vitro Flagellar Type III Protein Transport Assay Using Inverted Membrane Vesicles -- Molecular Simulation to Investigate Open‒Close Motion of a Flagellar Export Apparatus Protein FlhAC -- Live-Cell Imaging of the Assembly and Ejection Processes of the Bacterial Flagella by Fluorescence Microscopy -- Purification and CryoEM Image Analysis of the Bacterial Flagellar Filament -- Site-Specific Isotope Labeling of FliG for Studying Structural Dynamics Using Nuclear Magnetic Resonance Spectroscopy -- Site-Directed Crosslinking between Bacterial Flagellar Motor Proteins In Vivo -- Measurements of the Ion Channel Activity of the Transmembrane Stator Complex in the Bacterial Flagellar Motor -- Purification of the Na+-Driven PomAB Stator Complex and Its Analysis Using ATR-FTIR Spectroscopy -- Purification of Na+-Driven MotPS Stator Complexes and Single-Molecule Imaging by High-Speed Atomic Force Microscopy -- High-Resolution Rotation Assay of the Bacterial Flagellar Motor Near Zero Loads Using a Mutant Having a Rod-Like Straight Hook -- Live-Cell Fluorescence Imaging of Magnetosome Organelle for Magnetotaxis Motility -- Swarming Motility Assays in Salmonella -- Analysis of Adhesion and Surface Motility of a Spirochete Bacterium -- Force Measurement of Bacterial Swimming Using Optical Tweezers -- Archaella Isolation -- Direct Observation of Archaellar Motor Rotation by Single-Molecular Imaging Techniques -- In Situ Structure Determination of Bacterial Surface Nanomachines Using Cryo-Electron Tomography -- Twitching Motility Assays of Lysobacter enzymogenes OH11 Under a Light Microscope -- Live Cell Imaging of the Twitching Motility of Cyanobacteria by High-Resolution Microscopy -- Isolation and Visualization of Gliding Motility Machinery in Bacteroidota -- Live Cell Imaging of Gliding Motility of Flavobacterium johnsoniaeUnder High-Resolution Microscopy -- Social Motility Assays of Flavobacterium johnsoniae -- Visualization of Peptidoglycan Structures of Escherichia coli by Quick-Freeze Deep-Etch Electron Microscopy -- Purification and Structural Analysis of the Gliding Motility Machinery in Mycoplasma mobile -- Motility Assays of Mycoplasma mobile Under Light Microscopy -- Detection of Steps and Rotation in the Gliding Motility of Mycoplasma mobile -- Direct Measurement of Kinetic Force Generated by Mycoplasma -- Genetic Manipulation of Mycoplasma pneumonia -- Purification and ATPase Activity Measurement of Spiroplasma MreB -- Swimming Motility Assays of Spiroplasma -- Motility Assays of Chloroflexus.
520 _aThis detailed volume presents cutting-edge research protocols to study the structure and dynamics of bacterial and archaeal motility systems using bacterial genetics, molecular biology, biochemistry, biophysics, structural biology, cell biology, microscopy imaging, and molecular dynamics simulation. Beginning with a section on bacterial flagellar protein export and assembly, the book continues with chapters covering flagella-driven motility of bacteria, archaella-driven motility of archaea, type IV-driven twitching motility of bacteria, as well as adhesion-based gliding motility of bacteria and other unique motility systems. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Bacterialand Archaeal Motility is the ideal reference for researchers working in this vital area of microbiology.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aBacteria.
650 0 _aArchaebacteria.
650 1 4 _aBacteria.
650 2 4 _aArchaea.
700 1 _aMinamino, Tohru.
_eeditor.
_0(orcid)0000-0002-8175-1951
_1https://orcid.org/0000-0002-8175-1951
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aMiyata, Makoto.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aNamba, Keiichi.
_eeditor.
_0(orcid)0000-0003-2911-5875
_1https://orcid.org/0000-0003-2911-5875
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071630594
776 0 8 _iPrinted edition:
_z9781071630617
776 0 8 _iPrinted edition:
_z9781071630624
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2646
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-3060-0
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