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020 _a9781071628119
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024 7 _a10.1007/978-1-0716-2811-9
_2doi
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082 0 4 _a571.96028
_223
245 1 0 _aSignal Transduction Immunohistochemistry
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Alexander E. Kalyuzhny.
250 _a3rd ed. 2023.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2023.
300 _aXI, 328 p. 84 illus., 67 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2593
505 0 _aTop 5 Considerations When Choosing an Antibody -- Validating Antibody Specificities for Immunohistochemistry by Protein Blotting -- Recent Advances in Chromogens for Immunohistochemistry -- Overview of Imaging Techniques in Immunohistochemistry -- Visualizing Activated Myofibroblasts Resulting from Differentiation of Mesenchymal Stem Cells -- Clinical Applications of Flow Cytometry in Cancer Immunotherapies: From Diagnosis to Treatments -- Labeling of Phospho-Specific Antibodies with oYo-Link® Epitope Tags for Multiplex Immunostaining -- Multiplex Immunochromogenic Tissue Staining Employing Primary Antibodies from the Same Species -- 3D Imaging for Cleared Tissues and Thicker Samples on Confocal and Light-Sheet Microscopes -- Optical Tissue Clearing Enables Three-Dimensional Morphometry in Experimental Nerve Regeneration Research -- Protocol and Software for Automated Detection of Lysosome Active “Runs” and “Flights” with Wavelet Transform Approach -- Phenotyping of M1 and M2a Macrophages and Differential Expression of ACE-2 on Monocytes by Flow Cytometry: Impact of Cell Culture Conditions and Sample Processing -- In Situ Hybridization (ISH) Combined with Immunocytochemistry (ICC) Co-Detection of Phosphorylated EGFR in A431 Cultured Cells -- In Situ Hybridization (ISH) Combined with Immunohistochemistry (IHC) for Co-Detection of EGFR RNA and Phosphorylated EGFR Protein in Lung Cancer Tissue -- Multiplex Spatial Protein Detection by Combining Immunofluorescence with Immunohistochemistry -- Transcription Factor-Mediated Differentiation of Motor Neurons from Human Pluripotent Stem Cells -- Immunohistochemical Techniques for Phosphoproteins -- Visualizing Cofilin-Actin Filaments by Immunofluorescence and CryoEM: Essential Steps for Observing Cofilactin in Cells -- Cyclic Multiplex Fluorescent Immunohistochemistry Protocol to Phenotype Glial Cells in Human Formalin-Fixed Paraffin-Embedded Brain Sections -- Spatially Characterizing the Immune Contexture in Mouse Tissue Using Multiplex Immunohistochemistry -- Highly Multiplexed Immuno-Fluorescence Imaging for Quantitative Spatial Analysis in Tissue Samples with ChipCytometryTM.
520 _aThis new edition collects diverse protocols compiled by experts from different areas of research as well as by biotech researchers developing novel technologies in the area of immunohistochemistry (IHC). After a few chapters to help orient novices entering the biomedical arena, the book includes methods chapters covering multiplex IHC including fluorescence and chromogenic techniques, combination of IHC with In Situ Hybridization (ISH), wavelet transform approach for organelle tracking, transcription factors in human stem cells, differentiation of mesenchymal stem cells, 3D imaging, phenotyping of glial cells in the human brain, tissue fixation, permeabilization, and cryo-preservation, as well as other topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Signal Transduction Immunohistochemistry: Methods and Protocols, Third Edition aims to arm novices and experts with vital protocols they can use either as-is or tailor them for specific experimental needs.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aBiology
_xTechnique.
650 0 _aExperimental immunology.
650 0 _aTissues.
650 0 _aChemistry.
650 1 4 _aImmunological Techniques.
650 2 4 _aTissues.
700 1 _aKalyuzhny, Alexander E.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071628102
776 0 8 _iPrinted edition:
_z9781071628126
776 0 8 _iPrinted edition:
_z9781071628133
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2593
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-2811-9
912 _aZDB-2-PRO
999 _c5869
_d5869