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020 _a9781071624975
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024 7 _a10.1007/978-1-0716-2497-5
_2doi
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245 1 0 _aSpatial Genome Organization
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Tom Sexton.
250 _a1st ed. 2022.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2022.
300 _aXI, 333 p. 52 illus., 45 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
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490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2532
505 0 _aQuantitative Chromosome Conformation Capture (3C-qPCR) -- Detection of Allele-Specific 3D Chromatin Interactions Using High-Resolution In-Nucleus 4C-Seq -- Tough Tissue Hi-C -- Mapping Mammalian 3D Genomes by Micro-C -- Targeted Chromosome Conformation Capture (HiCap) -- Assessment of Higher-Order, Multi-Way Interactions with Tri-C -- Assessing Specific Networks of Chromatin Interactions with HiChIP -- Measuring Cytological Proximity of Chromosomal Loci to Defined Nuclear Compartments with TSA-Seq -- The High-Salt Recovered Sequence-Sequencing (HRS-seq) Method: Exploring Genome Association with Nuclear Bodies -- High-Throughput Preparation of Improved Single-Cell Hi-C Libraries Using an Automated Liquid Handling System -- Simultaneous Quantification of Spatial Genome Positioning and Transcriptomics in Single Cells with scDam&T-Seq -- High-Throughput DNA FISH (hiFISH) -- Versatile CRISPR-Based Method for Site-Specific Insertion of Repeat Arrays to Visualize Chromatin Loci in Living Cells -- CLOuD9:CRISPR-Cas9-Mediated Technique for Reversible Manipulation of Chromatin Architecture -- Acute Protein Depletion Strategies to Functionally Dissect the 3D Genome.
520 _aThis detailed volume explores a variety of cutting-edge techniques used to interrogate spatial genome organization. Beginning with a section covering the vital chromosome conformation capture (3C) technique, this collection continues with chapters on targeted Hi-C approaches, sequencing-based approaches to assess nuclear environment, as well as single-cell technologies to better characterize the heterogeneity and dynamics of nuclear architectures and approaches to visualize them by microscopy. Finally, in order to be able to ask functional questions about the role of spatial chromatin organization in genomic control, the last section provides methods for acute manipulations of chromatin architecture. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Spatial Genome Organization: Methods and Protocols is an ideal resource for researchers searching for the best techniques to address their own specific research questions.
650 0 _aBiology
_xTechnique.
650 0 _aGenomics.
650 1 4 _aGenomic Analysis.
650 2 4 _aGenomics.
700 1 _aSexton, Tom.
_eeditor.
_0(orcid)0000-0002-7824-1846
_1https://orcid.org/0000-0002-7824-1846
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071624968
776 0 8 _iPrinted edition:
_z9781071624982
776 0 8 _iPrinted edition:
_z9781071624999
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2532
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-2497-5
912 _aZDB-2-PRO
999 _c5866
_d5866