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020 _a9781071617830
_9978-1-0716-1783-0
024 7 _a10.1007/978-1-0716-1783-0
_2doi
050 4 _aQP361-430
050 4 _aQL931
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_2bicssc
072 7 _aSCI089000
_2bisacsh
072 7 _aPSAN1
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082 0 4 _a612.81046
_223
245 1 0 _aNeural Progenitor Cells
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Loic P. Deleyrolle.
250 _a2nd ed. 2022.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2022.
300 _aXI, 223 p. 55 illus., 41 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2389
505 0 _aCulturing Mouse Fetal Neural Precursor Cells in a Free-Floating Serum-Free Condition -- Isolation and Culture of Neural Stem/Progenitor Cells from the Postnatal Periventricular Region -- Isolation and Culture of Adult Hippocampal Precursor Cells as Free-Floating Neurospheres -- Isolate and Culture Neural Stem Cells from the Mouse Adult Spinal Cord -- Culturing and Expansion of “Clinical Grade” Neural Stem Cells from the Fetal Human Central Nervous System -- Microglia Isolation from Neural Stem Cell-Enriched Regions -- Microglial FACS Sorting for Robust RNA Recovery for Next Generation Sequencing -- Method for Isolating Extracellular Vesicles from Human Neural Stem Cells Expanded under Neurosphere Culture -- Isolating and Culturing of Precursor Cells from the Adult Human Brain -- Isolation and Culture of Precursor Cells from the Adult Human Spinal Cord -- Isolation and Enrichment of Defined Neural Cell Populations from Heterogeneous Neural Stem Cell Progeny -- Identifying Neural Progenitor Cells in the Adult Human Brain -- Neonatal Transplant in Hypoxic Injury -- Isolation and Purification of Self Renewable Human Neural Stem Cells from iPSCs for Cell Therapy in Experimental Model of Ischemic Stroke -- Generating Cerebral Organoids from Human Pluripotent Stem Cells -- Advancing Our Understanding of Brain Disorders: Research Using Postmortem Brain Tissue -- Biovalue in Human Brain Banking: Applications and Challenges for Research in Neurodegenerative Diseases.
520 _aThis updated edition collects cutting-edge techniques used to study neural stem and progenitor cells as well as the brain microenvironment. Featuring a wide range of technological advances in the study of neural stem cells, the volume highlights the promises of stem cell-based therapeutic applications for central nervous system ailments. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Neural Progenitor Cells: Methods and Protocols, Second Edition serves as an invaluable resource for the next generation of neuroscientists as they develop innovative experimental paradigms and progress toward therapeutic applications in the field of neurobiology.
650 0 _aNeurophysiology.
650 0 _aNeurons.
650 0 _aStem cells.
650 0 _aNeuroanatomy.
650 1 4 _aCellular Neuroscience.
650 2 4 _aStem Cell Biology.
650 2 4 _aNervous System.
700 1 _aDeleyrolle, Loic P.
_eeditor.
_0(orcid)0000-0002-1129-744X
_1https://orcid.org/0000-0002-1129-744X
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071617823
776 0 8 _iPrinted edition:
_z9781071617847
776 0 8 _iPrinted edition:
_z9781071617854
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2389
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-1783-0
912 _aZDB-2-PRO
999 _c5846
_d5846