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020 _a9781071612866
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024 7 _a10.1007/978-1-0716-1286-6
_2doi
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072 7 _aSCI007000
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082 0 4 _a572.7
_223
245 1 0 _aFlavins and Flavoproteins
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Maria Barile.
250 _a1st ed. 2021.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Springer,
_c2021.
300 _aXIII, 298 p. 69 illus., 41 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2280
505 0 _aSelection of Riboflavin Overproducing Strains of Lactic Acid Bacteria and Riboflavin Direct Quantification by Fluorescence -- Recent Advances in Construction of the Efficient Producers of Riboflavin and Flavin Nucleotides (FMN, FAD) in the Yeast Candida Famata -- Overexpression of Riboflavin Excretase Enhances Riboflavin Production in the Yeast Candida Famata -- Functional Study of the Human Riboflavin Transporter 2 Using Proteoliposomes System -- Heterologous Over-Expression of Human FAD Synthase Isoforms 1 and 2 -- Purification of Recombinant Human 6His-FAD Synthase (Isoform 2) and Quantitation of FAD / Protein Monomer Ratio by UV/Vis Spectra -- Continuous and Discontinuous Approaches to Study FAD Synthesis and Degradation Catalyzed by Purified Recombinant FAD Synthase or Cellular Fractions -- Redox Titration of Flavoproteins: An Overview -- Anaerobic Stopped-Flow Spectrophotometry with Photodiode Array Detection in the Pre-Steady State: An Application to Elucidate Oxido-Reduction Mechanisms in Flavoproteins -- Atomic Force Microscopy: Single Molecule Imaging and Force Spectroscopy in the Study of Flavoproteins Ligand Binding and Reaction Mechanisms -- Ligand Binding in Allosteric Flavoproteins: Part 1. Quantitative Analysis of the Interaction with NAD+ of the Apoptosis Inducing Factor (AIF) Harboring FAD in the Reduced State -- Ligand Binding in Allosteric Flavoproteins: Part 2. Quantitative Analysis of the Redox-Dependent Interaction of the Apoptosis-Inducing Factor (AIF) with Its Protein Partner -- Using D- and L-Amino Acid Oxidases to Generate the Imino Acid Substrate to Measure the Activity of the Novel Rid (Enamine/Imine Deaminase) Class of Enzymes -- The In Vitro Production of prFMN for Reconstitution of UbiD Enzymes -- Alcohol Oxidase from the Methylotrophic Yeast Ogataea polymorpha: Isolation, Purification, and Bioanalytical Application -- Flavocytochrome b2 of the Methylotrophic Yeast Ogataea polymorpha:Construction of Overproducers, Purification, and Bioanalytical Application -- Mammalian Flavoproteome Analysis Using Label-Free Quantitative Mass Spectrometry -- Alteration of Flavin Cofactor Homeostasis in Human Neuromuscular Pathologies.
520 _aThis book of protocols is devoted to the yellow coenzymes derived from riboflavin or vitamin B2 and to the hundreds of enzymes whose functionality depends on them, and represents a compendium of techniques for working with flavoproteins or with the wide spectrum of functions that flavoproteins can drive in the cells. Starting with Rf production in microorganisms and the chemical, optical, and redox properties of these fascinating molecules and moving along to the variety and the peculiarity of some single flavoenzymes, the volume explores the complexity of functions and distribution of these molecules in the cell. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Flavin and Flavoproteins: Methods and Protocols serves as an ideal guide for protein chemists interested in purifying and characterizing flavoproteins, as well as microbiologists, physiologists, and clinicians, who wish to further study problems connected with flavoproteins.
650 0 _aEnzymology.
650 0 _aChemistry.
650 1 4 _aEnzymology.
650 2 4 _aChemistry.
700 1 _aBarile, Maria.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071612859
776 0 8 _iPrinted edition:
_z9781071612873
776 0 8 _iPrinted edition:
_z9781071612880
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2280
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-1286-6
912 _aZDB-2-PRO
999 _c5827
_d5827