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024 7 _a10.1007/978-1-0716-1008-4
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245 1 0 _aMouse Genetics
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Shree Ram Singh, Robert M. Hoffman, Amit Singh.
250 _a2nd ed. 2021.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2021.
300 _aXI, 217 p. 47 illus., 41 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
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490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2224
505 0 _aGeneration of Mouse Model (KI & CKO) via Easi-CRISPR -- Analysis of Gene Expression Using lacZ Reporter Mouse Lines -- Linear Density Sucrose Gradients to Study Mitoribosomal Biogenesis in Tissue-Specific Knockout Mice -- Mouse Models for Studying Hippocampal Adult Neural Stem Cell Biology -- Retina as a Model to Study In Vivo Transmission of α-Synuclein in the A53T Mouse Model of Parkinson’s Disease -- Promoting Pro-Endocrine Differentiation and Graft Maturation Following Surgical Resection of the Mouse Pancreas -- Color-Coded Imaging of Cancer and Stromal Cells Interaction in the Pancreatic Cancer Tumor Microenvironment (TME) -- Generating Ins2+/-/miR-133aTg Mice to Model miRNA-Driven Cardioprotection of Diabetic Hearts in Humans -- Generating a Podocyte-Specific FcRn Knockout Mouse -- Mouse Models of Colitis-Associated Colon Cancer -- Colon Cancer Tumor Model Base on Colonoscopy Injection -- Generation of Transgenic Fluorescent Reporter Lines for Studying Hematopoietic Development in the Mouse -- Gene Inactivation in Adult Long Term-Hematopoietic Stem Cells Using Inducible Mouse Models -- Hematological Humanization of Immune Deficient Mice -- Antisense Oligonucleotide Treatment in a Humanized Mouse Model of Duchenne Muscular Dystrophy and Highly Sensitive Detection of Dystrophin Using Western Blotting.
520 _aThis fully updated edition provides selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish the manipulation of genes of interest, tracing cell lineages, and modeling human diseases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Mouse Genetics: Methods and Protocols, Second Edition delivers fundamental techniques and protocols to geneticists, molecular biologists, cell and developmental biologists, students, and postdoctoral fellows working in the various disciplines of genetics, developmental biology, mouse genetics, and modeling human diseases.
650 0 _aBiology
_xTechnique.
650 0 _aMedicine
_xResearch.
650 0 _aBiology
_xResearch.
650 0 _aZoology.
650 1 4 _aBiological Techniques.
650 2 4 _aBiomedical Research.
650 2 4 _aZoology.
700 1 _aSingh, Shree Ram.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aHoffman, Robert M.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aSingh, Amit.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071610077
776 0 8 _iPrinted edition:
_z9781071610091
776 0 8 _iPrinted edition:
_z9781071610107
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2224
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-1008-4
912 _aZDB-2-PRO
999 _c5825
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