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020 _a9781071610565
_9978-1-0716-1056-5
024 7 _a10.1007/978-1-0716-1056-5
_2doi
050 4 _aQH573-671
072 7 _aPSF
_2bicssc
072 7 _aSCI017000
_2bisacsh
072 7 _aPSF
_2thema
082 0 4 _a571.6
_223
245 1 0 _aPericytes
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Bruno M. Péault.
250 _a1st ed. 2021.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2021.
300 _aXI, 192 p. 29 illus., 26 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2235
505 0 _aVisualizing Pericyte Mimicry of Angiotropic Melanoma by Direct Labeling of the Angioarchitecture -- Transplantation of Purified Human Pericytes for Myocardial Repair in SCID-hu Mice -- Assessment of Pericyte Phenotype by Flow Cytometry -- Characterization of Pericyte Phenotype In Vivo by Immunohistochemistry -- A Metabolomics Based Approach to Identify Lineage Guiding Molecules in Pericyte Cultures -- Pericyte Ontogeny: The Use of Chimeras to Track a Cell Lineage of Diverse Germ Line Origins -- Imaging Pericytes and the Regulation of Cerebral Blood Flow -- Derivation of Pericytes from Human Pluripotent Stem Cells -- Assessing the Bone-Forming Potential of Pericytes -- Manipulating Pericyte Function with MicroRNAs -- Isolation of Mesoangioblasts: A Subset of Pericytes with Strong Myogenic Potential from Mammals -- Role of Pericytes in the Development of the Renin/Angiotensin System: Induction of Functional Renin in Cultures of Pericytes -- Metastatic Dissemination Mimicked in a Multicellular Transwell Assay.
520 _aThis volume explores the techniques used to study human and animal pericytes. Chapters in this volume cover a range of topics such as pericyte dissociation from tissues, and analysis by immunohistochemistry and flow cytometry; avian chimaeras to investigate the embryonic development of cell lineage; pericyte purification by fluorescence activated cell sorting and ensuing culture; tissue turnover and regeneration; and the dynamic study of pericyte involvement in cancer dissemination, in situ, and in culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Pericytes: Methods and Protocols is a valuable resource that helps researchers define the biologic identity, functions, andpotentials of a cell lineage of increasing interest and therapeutic significance. .
650 0 _aCytology.
650 1 4 _aCell Biology.
700 1 _aPéault, Bruno M.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071610558
776 0 8 _iPrinted edition:
_z9781071610572
776 0 8 _iPrinted edition:
_z9781071610589
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2235
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-1056-5
912 _aZDB-2-PRO
999 _c5824
_d5824