000 04573nam a22004935i 4500
001 978-1-0716-1142-5
003 DE-He213
005 20260610083636.0
007 cr nn 008mamaa
008 210122s2021 xxu| s |||| 0|eng d
020 _a9781071611425
_9978-1-0716-1142-5
024 7 _a10.1007/978-1-0716-1142-5
_2doi
050 4 _aQH345
050 4 _aQP751-752
072 7 _aPSBH
_2bicssc
072 7 _aSCI007000
_2bisacsh
072 7 _aPSE
_2thema
082 0 4 _a572.57
_223
245 1 0 _aPhosphoinositides
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Roberto J. Botelho.
250 _a1st ed. 2021.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2021.
300 _aXI, 239 p. 50 illus., 43 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2251
505 0 _aSimultaneous Detection of Phosphoinositide Lipids by Radioactive Metabolic Labeling -- Label-Free Quantification of Phosphoinositides in Drosophila by Mass Spectrometry -- Profiling of Phosphoinositide Molecular Species in Resting or Activated Human or Mouse Platelets by a LC-MS Method -- Quantification of Genetically-Encoded Lipid Biosensors -- Detection of Plasma Membrane Phosphoinositide Dynamics Using Genetically-Encoded Fluorescent Protein Probes -- Imaging the Nanoscale Distribution of Phosphoinositides in the Cell Plasma Membrane with Single-Molecule Localization Super-Resolution Microscopy -- Induced Dimerization Tools to Deplete Specific Phosphatidylinositol Phosphates -- A Real-Time Phosphatidylinositol 4-Phosphate 5-Kinase Assay Using Fluorescence Spectroscopy -- Assessing In Situ Phosphoinositide-Protein Interactions through Fluorescence Proximity Ligation Assay in Cultured Cells -- Characterization of Protein-Phospholipid/Membrane Interactions Using a “Membrane-on-a-Chip” Microfluidic System -- Exploring Phosphoinositide Binding Using Native Mass Spectrometry -- Liposome-Based Methods to Study Protein-Phosphoinositide Interaction -- Liposome-Based Methods to Study GTPases Activation by Phosphoinositides -- Liposome Co-Sedimentation and Co-Flotation Assays to Study Lipid-Protein Interactions -- Characterization of PROPPIN-Phosphoinositide Binding by Stopped-Flow Fluorescence Spectroscopy -- Fluorescence Assays to Study Membrane Penetration of Proteins -- Fluorogenic XY-69 in Lipid Vesicles for Measuring Activity of Phospholipase C Isozymes.
520 _aThis detailed book examines experimental approaches used to investigate the regulation and function of phosphoinositides (PtdInsP), rare eukaryotic phospholipids with a broad role in biological processes such as signal transduction, cell migration and adhesion, cell growth, subcellular organization, and membrane trafficking. The combination of complementary biochemical, mass spectrometry, and imaging methods are instrumental for the detection and quantification of PtdInsP species, as well as induced dimerization methods, affinity precipitation or co-sedimentation with liposomes, protein insertion within lipid bilayers, and enzymatic assays, or through emerging methods like native mass spectrometry and microfluidics, all of which are touched upon in this volume. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Phosphoinositides: Methods and Protocols provides detailed methodology for both specialist and novice researchers on a variety of complementary methods that have been instrumental in dissecting the regulation, dynamics, and function of PtdInsPs.
650 0 _aLipids.
650 0 _aCytology.
650 1 4 _aLipidology.
650 2 4 _aCell Biology.
700 1 _aBotelho, Roberto J.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071611418
776 0 8 _iPrinted edition:
_z9781071611432
776 0 8 _iPrinted edition:
_z9781071611449
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2251
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-1142-5
912 _aZDB-2-PRO
999 _c5823
_d5823