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020 _a9781071608340
_9978-1-0716-0834-0
024 7 _a10.1007/978-1-0716-0834-0
_2doi
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072 7 _aMFN
_2bicssc
072 7 _aMED107000
_2bisacsh
072 7 _aMFN
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082 0 4 _a616.042
_223
245 1 0 _aMitochondrial Gene Expression
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Michal Minczuk, Joanna Rorbach.
250 _a1st ed. 2021.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2021.
300 _aXII, 333 p. 59 illus., 40 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2192
505 0 _aIn vitro Analysis of mtDNA Replication -- In vivo Analysis of mtDNA Replication at the Single Molecule Level and with High Resolution -- In Vitro Reconstitution of Human Mitochondrial Transcription -- Investigating Mitochondrial Transcriptomes and RNA Processing using Circular RNA Sequencing -- Detection of 5-formylcytosine in Mitochondrial Transcriptome -- Visualization of Mitochondrial RNA Granules in Cultured Cells using 5-Bromouridine Labeling -- Quantitative Proteomics in Drosophila with Holidic Stable Isotope Labeling of Amino Acids in Fruit Flies (SILAF) -- Mass Spectrometric Analysis of Mitochondrial RNA Modifications -- mito-Ψ-Seq: A High-throughput Method for Systematic Mapping of Pseudouridine within Mitochondrial RNA -- High Throughput Detection of mtDNA Mutations Leading to tRNA Processing Errors -- High-throughput Measurement of Mitochondrial RNA Turnover in Human Cultured Cells -- RNA Crosslinking to Analyze the Mitochondrial RNA-binding Proteome -- Visualizing Mitochondrial Ribosomal RNA and Mitochondrial Protein Synthesis in Human Cell Lines -- Mitoribosome Profiling From Human Cell Culture - A High Resolution View of Mitochondrial Translation -- Application of cryo-EM for Visualization of Mitoribosomes -- Sucrose Gradient Sedimentation Analysis of Mitochondrial Ribosomes -- The Analysis of Yeast Mitochondrial Translation -- In situ Studies of Mitochondrial Translation by Cryoelectron Tomography -- Mitochondrial Complexome Profiling -- Blue Native Electrophoresis to Study the Oxphos Complexes -- Identification of Putative Mitochondrial Protease Substrates. .
520 _aThis volume details the most recent advancements in the field of mitochondrial gene expression. Chapters guide readers through methods and protocols on mtDNA replication, transcription, and translation to membrane insertion of the mtDNA-encoded protein products. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mitochondrial Gene Expression: Methods and Protocols aims to provide complementary approaches and practical guidelines. Chapter 7 is available Open Access under a Creative Commons Attribution 4.0 International License via link.springer.com.
650 0 _aMedical genetics.
650 1 4 _aMedical Genetics.
700 1 _aMinczuk, Michal.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aRorbach, Joanna.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071608333
776 0 8 _iPrinted edition:
_z9781071608357
776 0 8 _iPrinted edition:
_z9781071608364
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2192
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-0834-0
912 _aZDB-2-PRO
999 _c5818
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