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020 _a9781071609583
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024 7 _a10.1007/978-1-0716-0958-3
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072 7 _aPSC
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072 7 _aSCI072000
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072 7 _aPSC
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082 0 4 _a571.8
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245 1 0 _aEpigenetic Reprogramming During Mouse Embryogenesis
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Katia Ancelin, Maud Borensztein.
250 _a1st ed. 2021.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2021.
300 _aXI, 318 p. 74 illus., 58 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
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490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2214
505 0 _aEx Vivo Culture for Preimplantation Mouse Embryo to Analyse Pluripotency -- Microfabricated Device for High Resolution Imaging of Preimplantation Embryos -- In Vitro Culture of Mouse Blastocyst to the Egg Cylinder Stage via Mural Trophectoderm Excision -- Spatially Organized Differentiation of Mouse Pluripotent Stem Cells on Micropatterned Surfaces -- Mouse Primordial Germ Cells: In Vitro Culture and Conversion to Pluripotent Stem Cell Lines -- Generation of Primordial Germ Cell-Like Cells in Small and Large Scales -- Genome-Scale CRISPR Screening for Regulators of Cell Fate Transitions -- Somatic Reprogramming by Nuclear Transfer -- Targeted Transgenic Mice using CRISPR/Cas9 Technology -- Whole-Mount Immunofluorescence Staining of Early Mouse Embryos -- Investigating the Inner Cell Mass of the Mouse Blastocyst by Combined Immunofluorescence Staining and RNA Fluorescent In Situ Hybridization -- Mapping of Chromosome Territories by 3D-Chromosome Painting during Early Mouse Development -- Deciphering the Early Mouse Embryo Transcriptome by Low-Input RNA-seq -- Studying DNA Methylation Genome-Wide by Bisulfite Sequencing from Low Amounts of DNA in Mammals -- Profiling DNA Methylation Genome-Wide in Single Cells -- Tracking Histone Modifications in Embryos and Low Input Samples using Ultrasensitive STAR ChIP-seq -- Chromatin Profiling in Mouse Embryonic Germ Cells by CUT&RUN -- DamID to Map Genome-Protein Interactions in Pre-Implantation Mouse Embryos -- Understanding Chromosome Structure during Early Mouse Development by Single-Cell Hi-C Analysis -- Bioinformatic Analysis of Single Cell Hi-C Data from Early Mouse Embryo.
520 _aThis volume explores the latest techniques used to study and understand chromatin reprogramming in embryos and germ cells. Various culture systems are presented, which consist of invaluable tools to investigate many developmental processes. This book also looks at methods for direct examination of DNA, RNA, and proteins in embryos, along with low-input and single-cell assays for exploring these features at the genome-wide scale. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Epigenetics Reprogramming During Mouse Embryogenesis: Methods and Protocols is a valuable resource for any scientist and researcher looking to make new discoveries in this fascinating field of chromatin reprogramming. .
650 0 _aDevelopmental biology.
650 0 _aGenetics.
650 0 _aBiology
_xTechnique.
650 1 4 _aDevelopmental Biology and Stem Cells.
650 2 4 _aGenetics and Genomics.
650 2 4 _aBiological Techniques.
700 1 _aAncelin, Katia.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aBorensztein, Maud.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071609576
776 0 8 _iPrinted edition:
_z9781071609590
776 0 8 _iPrinted edition:
_z9781071609606
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2214
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-0958-3
912 _aZDB-2-PRO
999 _c5817
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