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020			_a9781493996704 _9978-1-4939-9670-4
024	7		_a10.1007/978-1-4939-9670-4 _2doi
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050		4	_aQH315-320
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082	0	4	_a610.72 _223
245	1	0	_aOligonucleotide-Based Therapies _h[electronic resource] : _bMethods and Protocols / _cedited by Olof Gissberg, Rula Zain, Karin E. Lundin.
250			_a1st ed. 2019.
264		1	_aNew York, NY : _bSpringer New York : _bImprint: Humana, _c2019.
300			_aXI, 344 p. 69 illus., 46 illus. in color. _bonline resource.
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
347			_atext file _bPDF _2rda
490	1		_aMethods in Molecular Biology, _x1940-6029 ; _v2036
505	0		_aChemical Development of Therapeutic Oligonucleotides -- Tissue-Specific Delivery of Oligonucleotides -- Designing siRNA and Evaluating Its Effect on RNA Targets Using qPCR and Western Blot -- Antisense Oligonucleotides for Splice Modulation: Assessing Splice Switching Efficacy -- Assessing Oligonucleotide Binding to Double-Strand DNA -- Modelling and Simulation of Oligonucleotide Hybrids: Outlining a Strategy -- Characterization of Peptide/Oligonucleotide Complexes Using Electron Microscopy, Dynamic Light Scattering, and Protease Resistance Assay -- Click-Shielded and Targeted Lipopolyplexes -- Attachment of Peptides to Oligonucleotides on Solid Support Using Copper(I)-Catalyzed Huisgen 1,3-Dipolar Cycloaddition -- Characterizing Oligonucleotide Uptake in Cultured Cells: A Case Study Using AS1411 Aptamer -- Measuring the Action of Oligonucleotide Therapeutics in the Lung at the Cell Type-Specific Level by Tissue Disruption and Cell Sorting (TDCS) -- Delivery of Antisense Oligonucleotides Mediatedby a Hydrogel System: In Vitro and In Vivo Application in the Context of Spinal Cord Injury -- Evaluation of Cell-Penetrating Peptide Delivery of Antisense Oligonucleotides for Therapeutic Efficacy in Spinal Muscular Atrophy -- Injection and Infusion of Compounds to the Central Nervous System -- In Vitro Assessment of the Hepatotoxicity Potential of Therapeutic Oligonucleotides -- Identifying Suitable Target Regions and Analyzing Off-Target Effects -- RACE-SEQ and Population-Wide Polymorphism Susceptibility Testing for Endonucleolytically Active, RNA-Targeting Therapeutics -- Studying the Biotransformation of Phosphorothioate-Containing Oligonucleotide Drugs by LC-MS -- ADME: Assessing Pharmacokinetic-Pharmacodynamic Parameters of Oligonucleotides.
520			_aThis book provides a collection of comprehensive, up-to-date, and broadly applicable guides to the research and development fields of oligonucleotide (ON) therapeutics. Covering topics from the study of antisense and anti-gene effects to oligonucleotides in the context of drug discovery and development, the volume explores a wide-ranging and useful spectrum of methods and protocols needed to take full advantage of therapeutic applications involving ONs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Oligonucleotide-Based Therapies: Methods and Protocols aims to be a great aid in the laboratory as well as an ideal reference guide when designing antisense and anti-gene oligonucleotides for therapeutic applications.
650		0	_aMedicine _xResearch.
650		0	_aBiology _xResearch.
650		0	_aMedical genetics.
650		0	_aPharmacology.
650	1	4	_aBiomedical Research.
650	2	4	_aMedical Genetics.
650	2	4	_aPharmacology.
700	1		_aGissberg, Olof. _eeditor. _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aZain, Rula. _eeditor. _0(orcid)0000-0001-8327-846X _1https://orcid.org/0000-0001-8327-846X _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aLundin, Karin E. _eeditor. _0(orcid)0000-0002-1489-3028 _1https://orcid.org/0000-0002-1489-3028 _4edt _4http://id.loc.gov/vocabulary/relators/edt
710	2		_aSpringerLink (Online service)
773	0		_tSpringer Nature eBook
776	0	8	_iPrinted edition: _z9781493996698
776	0	8	_iPrinted edition: _z9781493996711
776	0	8	_iPrinted edition: _z9781493996728
830		0	_aMethods in Molecular Biology, _x1940-6029 ; _v2036
856	4	0	_uhttps://doi.org/10.1007/978-1-4939-9670-4
912			_aZDB-2-PRO
999			_c5802 _d5802