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020 _a9781493994205
_9978-1-4939-9420-5
024 7 _a10.1007/978-1-4939-9420-5
_2doi
050 4 _aRM300-666
072 7 _aMMG
_2bicssc
072 7 _aMED071000
_2bisacsh
072 7 _aMKG
_2thema
082 0 4 _a615
_223
245 1 0 _aExperimental Cholestasis Research
_h[electronic resource] /
_cedited by Mathieu Vinken.
250 _a1st ed. 2019.
264 1 _aNew York, NY :
_bSpringer New York :
_bImprint: Humana,
_c2019.
300 _aXIII, 399 p. 74 illus., 60 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1981
505 0 _aMechanisms of Drug-Induced Cholestasis -- LC-MS/MS Analysis of Bile Acids in In Vitro Samples -- Pipe-3D: A Pipeline Based on Immunofluorescence, 3D Confocal Imaging, Reconstructions, and Morphometry for Biliary Network Analysis in Cholestasis -- Vesicle-Based and Hepatocyte-Based Assays for Identification of Drug Candidates Inhibiting BSEP Function -- The C-DILI™ Assay: An Integrated In Vitro Approach to Predict Cholestatic Hepatotoxicity -- In Vivo Measurement of Hepatic Drug Transporter Inhibition with Radiolabeled Bile Acids -- Measuring the Impact of Bile Acids on the Membrane Order of Primary Hepatocytes and Isolated Mitochondria by Fluorescence Imaging and Spectroscopy -- The Role and Study of Mitochondrial Impairment and Oxidative Stress in Cholestasis -- Measuring Apoptosis and Necrosis in Cholestatic Liver Injury -- Necrosome Formation and Necroptosis in Experimental Cholestasis -- Proteomics in Primary Biliary Cholangitis -- Transcriptomics Analysis of Cholestatic Compounds In Vitro -- Systems Microscopy of Stress Response Pathways in Cholestasis Research -- Mouse Models for Diseases in the Cholangiocyte Lineage -- Common Bile Duct Ligation as Model for Secondary Biliary Cirrhosis -- 3,5-Diethoxycarbonyl-1,4-Dihydrocollidine Diet: A Rodent Model in Cholestasis Research -- A Rotavirus-Induced Mouse Model to Study Biliary Atresia and Neonatal Cholestasis -- Zebrafish as a Model to Study Cholestatic Liver Diseases -- Set-Up and Use of HepaRG Cells in Cholestasis Research -- Organotypic 3D HepaRG Liver Model for Assessment of Drug-Induced Cholestasis -- Establishment of Sandwich Cultures of Primary Human Hepatocytes -- Detection of Drug-Induced Cholestasis Potential in Sandwich-Cultured Human Hepatocytes -- Ex Vivo Model in Cholestasis Research -- Patient-Derived Organoids from Human Bile: An In Vitro Method to Study Cholangiopathies -- Use of Biliary Organoids in Cholestasis Research -- In Silico Approaches to Predict Drug-Transporter Interaction Profiles: Data Mining, Model Generation, and Link to Cholestasis.
520 _aThis detailed book provides a state-of-the-art compilation of protocols relevant for the experimental study of cholestasis, each written by internationally recognized research teams that have built up long-standing expertise regarding one or more aspects of experimental cholestasis research. The first half of the collection examines methods targeted towards studying established and new mechanisms of cholestasis, while the second half explores experimental models and tools to investigate mechanisms of cholestasis and/or to predict cholestatic properties of drugs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Experimental Cholestasis Research serves as an ideal guide for basic andapplied researchers pursuing this vital area of research in pharmacology and toxicology.
650 0 _aPharmacology.
650 0 _aInternal medicine.
650 1 4 _aPharmacology.
650 2 4 _aInternal Medicine.
700 1 _aVinken, Mathieu.
_eeditor.
_0(orcid)0000-0001-5115-8893
_1https://orcid.org/0000-0001-5115-8893
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781493994199
776 0 8 _iPrinted edition:
_z9781493994212
776 0 8 _iPrinted edition:
_z9781493994229
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1981
856 4 0 _uhttps://doi.org/10.1007/978-1-4939-9420-5
912 _aZDB-2-PRO
999 _c5706
_d5706