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001 978-1-4614-9408-9
003 DE-He213
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020 _a9781461494089
_9978-1-4614-9408-9
024 7 _a10.1007/978-1-4614-9408-9
_2doi
050 4 _aQK
072 7 _aPST
_2bicssc
072 7 _aSCI011000
_2bisacsh
072 7 _aPST
_2thema
082 0 4 _a580
_223
245 1 0 _aFlower Development
_h[electronic resource] :
_bMethods and Protocols /
_cedited by José Luis Riechmann, Frank Wellmer.
250 _a1st ed. 2014.
264 1 _aNew York, NY :
_bSpringer New York :
_bImprint: Humana,
_c2014.
300 _aXIV, 475 p. 64 illus., 31 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1110
505 0 _aFlower Development in Arabidopsis - There is More to it than Learning your ABCs -- Flower Development in the Asterid Lineage -- Grass Flower Development -- Flower Diversity and Angiosperm Diversification -- Flower Development - Open Questions and Future Directions -- Genetic Screens for Floral Mutants in Arabidopsis thaliana: Enhancers and Suppressors -- Genetic and Phenotypic Analysis of Shoot Apical and Floral Meristem Development -- Genetic and Phenotypic Analyses of Petal Development in Arabidopsis -- Cell Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsis and Rice -- Molecular Cell Biology of Male Meiotic Chromosomes and Isolation of Male Meiocytes in Arabidopsis thaliana -- Genetic and Phenotypic Analyses of Carpel Development in Arabidopsis -- Microscopic Analysis of Arabidopsis Ovules -- Scanning Electron Microscopy Analysis of Floral Development -- Detection of mRNA Expression Patterns by Non-radioactive in situ Hybridization on Histological Sections of Floral Tissue -- The GUS Reporter System in Flower Development Studies -- A Floral Induction System for the Study of Early Arabidopsis Flower Development -- Fluorescence Activated Cell Sorting of Shoot Apical Meristem Cell Types -- Translating Ribosome Affinity Purification (TRAP) for Cell-specific Translation Profiling in Developing Flowers -- Laser-assisted Microdissection Applied to Floral Tissues -- Identification of Arabidopsis Knock-out Lines for Genes of Interest -- Gene Expression Analysis by Quantitative Real-time PCR for Floral Tissues -- Misexpression Approaches for the Manipulation of Flower Development -- Next Generation Sequencing Applied to Flower Development: RNA-Seq -- Next-Generation Sequencing Applied to Flower Development: ChIP-Seq -- Live-Imaging of the Arabidopsis Inflorescence Meristem -- Gene Regulatory Network Models for Floral Organ Determination.
520 _aIn Flower Development: Methods and Protocols, researchers in the field detail protocols for  experimental approaches that are currently used to study the formation of flowers, from genetic methods and phenotypic analyses, to genome-wide experiments, modeling, and system-wide approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls     Authoritative and practical, Flower Development: Methods and Protocols  is an essential guide for plant developmental biologists, from the novice to the experienced researcher, and for those considering venturing into the field.
650 0 _aBotany.
650 0 _aPlant genetics.
650 1 4 _aPlant Science.
650 2 4 _aPlant Genetics.
700 1 _aRiechmann, José Luis.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aWellmer, Frank.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781461494072
776 0 8 _iPrinted edition:
_z9781461494096
776 0 8 _iPrinted edition:
_z9781493943401
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1110
856 4 0 _uhttps://doi.org/10.1007/978-1-4614-9408-9
912 _aZDB-2-PRO
999 _c5675
_d5675