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001 978-1-59259-628-7
003 DE-He213
005 20260610083633.0
007 cr nn 008mamaa
008 100301s1992 xxu| s |||| 0|eng d
020 _a9781592596287
_9978-1-59259-628-7
024 7 _a10.1385/0896032140
_2doi
050 4 _aRC321-580
072 7 _aPSAN
_2bicssc
072 7 _aSCI089000
_2bisacsh
072 7 _aPSAN
_2thema
082 0 4 _a612.8
_223
245 1 0 _aPractical Cell Culture Techniques
_h[electronic resource] /
_cedited by Alan A. Boulton, Glen B. Baker, Wolfgang Walz.
250 _a1st ed. 1992.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c1992.
300 _aXIX, 392 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aNeuromethods,
_x1940-6045 ;
_v23
505 0 _aBasic Techniques for Cell Culturing -- Identification of Cell Types in Neural Cultures -- Environmental Influences on Cells in Culture -- Detection and Analysis of Growth Factors Affecting Neural Cells -- The Role of Cell Adhesion Molecules in Neurite Growth -- Three-Dimensional Organ Culture Systems -- Organotypic Slice Explant Roller-Tube Cultures -- Neurons -- Astrocytes -- Oligodendrocytes -- Brain Capillaries.
520 _aMost cells will survive removal from the natural mic- environment of their in vivo tissue and placement into a sterile culture dish under optimal conditions. Not only do they survive, but they also multiply and express differen- ated properties in such a culture dish. A few cells do this in suspension, but most will need some kind of mechanical support substituting for their natural connections with other cells. The surface of a culture dish that might have to be coated is usually sufficient. The recent trend to standa- ization of conditions and the existence of commercial ent- prises with adequate funds and specializing in the needs of scientists were responsible for the tremendous proliferation of cell culture techniques in all fields of research in the last 20 years. No longer does a scientist have to concentrate all his/her efforts on that technology; the new trends make it feasible to employ cell culture techniques as only one of the many methods available in a small corner of a larger research laboratory. Some areas of research depend more heavily than others on cell culture techniques. Neuroscience is one of the areas that has developed hand in hand with the prol- eration of cell culture methodology. Molecular biological aspects, cell differentiation and development, neurophy- ological and neurochemical studies, as well as investigations into the nature of various diseases are now to a large extent dependent on the use of cell cultures.
532 8 _aAccessibility summary: This PDF is not accessible. It is based on scanned pages and does not support features such as screen reader compatibility or described non-text content (images, graphs etc). However, it likely supports searchable and selectable text based on OCR (Optical Character Recognition). Users with accessibility needs may not be able to use this content effectively. Please contact us at accessibilitysupport@springernature.com if you require assistance or an alternative format.
532 8 _aInaccessible, or known limited accessibility
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aNeurosciences.
650 1 4 _aNeuroscience.
700 1 _aBoulton, Alan A.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aBaker, Glen B.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aWalz, Wolfgang.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896033481
776 0 8 _iPrinted edition:
_z9780896032149
776 0 8 _iPrinted edition:
_z9781489940209
830 0 _aNeuromethods,
_x1940-6045 ;
_v23
856 4 0 _uhttps://doi.org/10.1385/0896032140
912 _aZDB-2-PRO
999 _c5674
_d5674