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020 _a9781071631317
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024 7 _a10.1007/978-1-0716-3131-7
_2doi
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072 7 _aPST
_2bicssc
072 7 _aSCI011000
_2bisacsh
072 7 _aPST
_2thema
082 0 4 _a580
_223
245 1 0 _aPlant Genome Engineering
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Bing Yang, Wendy Harwood, Qiudeng Que.
250 _a1st ed. 2023.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2023.
300 _aXV, 388 p. 70 illus., 65 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2653
505 0 _aPAM-Less CRISPR-SpRY Genome Editing in Plants -- Type I-D CRISPR System-Mediated Genome Editing in Plants -- CRISPR/LbCas12a-Mediated Genome Editing in Soybean -- Base Editing in Poplar through an Agrobacterium-Mediated Transformation Method -- Genetic Engineering of Potato (Solanum tuberosum) Chloroplasts using the Small Synthetic Plastome “Mini-Synplastome” -- Designing Guide-RNA for Generating Premature Stop Codons for Gene Knockout using CRISPR-BETS -- Construction of CRISPR/Cas9 Multiplex Genome Editing System in Rice -- Use of Fluorescent Protein Reporters for Assessing and Detecting Genome Editing Reagents and Transgene Expression in Plants -- Automated, High-Throughput Protoplast Transfection for Gene Editing and Transgene Expression Studies -- Delivery of CRISPR-Cas12a Ribonucleoprotein Complex for Genome Editing in an Embryogenic Citrus Cell Line -- Transgene-Free Genome Editing in Nicotiana benthamiana with CRISPR/Cas9 Delivered by a Rhabdovirus Vector -- Ribonucleoprotein (RNP)-Mediated Targeted Mutagenesis in Barley (Hordeum vulgare L.) -- Ribonucleoprotein (RNP)-Mediated Allele Replacement in Barley (Hordeum vulgare L.) Leaves -- Genome Editing in Chlamydomonas reinhardtii Using Cas9-gRNA Ribonucleoprotein Complex: A Step-by-Step Guide -- Highly Efficient Gene Knockout in Medicago truncatula Genotype R108 using CRISPR-Cas9 System and an Optimized Agrobacterium Transformation Method -- Efficient Targeted Mutagenesis in Brassica Crops using CRISPR/Cas Systems -- Introduction of Genome Editing Reagents and Genotyping of Derived Edited Alleles in Soybean (Glycine max (L.) Merr.) -- A CRISPR/Cas9 Protocol for Target Gene Editing in Barley -- Targeted Insertion in Nicotiana benthamiana Genomes via Protoplast Regeneration -- Stepwise Optimization of Real-Time RT-PCR Analysis -- CRISPR/Cas9 Technology for Potato Functional Genomics and Breeding -- Recent Advances in Engineering of In Vivo Haploid Induction Systems.
520 _aThis volume provides readers with a collection of the latest protocols used to study plant genome editing and trait engineering. The chapters in this book are organized into five parts: genome engineering systems; machinery design and validation; delivery tools; generation and analysis of engineering materials; and crop genome engineering applications. The chapters cover topics such as methods of applying the popular CRISPR-Cas9 or CRISPR-Cas12 systems for editing genomes in different crop species, the use of small synthetic plastome for potato genome engineering, and the use of CRISPR-Cas9 for algal cell genome engineering. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Plant Genome Engineering: Methods and Protocols is a valuable tool for researchers interested in learning more about this developing and important field.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aBotany.
650 0 _aPlant genetics.
650 1 4 _aPlant Science.
650 2 4 _aPlant Genetics.
700 1 _aYang, Bing.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aHarwood, Wendy.
_eeditor.
_0(orcid)0000-0002-4323-9009
_1https://orcid.org/0000-0002-4323-9009
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aQue, Qiudeng.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071631300
776 0 8 _iPrinted edition:
_z9781071631324
776 0 8 _iPrinted edition:
_z9781071631331
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2653
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-3131-7
912 _aZDB-2-PRO
999 _c5665
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