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020 _a9781071625699
_9978-1-0716-2569-9
024 7 _a10.1007/978-1-0716-2569-9
_2doi
050 4 _aRC321-580
072 7 _aPSAN
_2bicssc
072 7 _aSCI089000
_2bisacsh
072 7 _aPSAN
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082 0 4 _a612.8
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245 1 0 _aTranslational Research Methods in Neurodevelopmental Disorders
_h[electronic resource] /
_cedited by Stéphane Martin, Frédéric Laumonnier.
250 _a1st ed. 2022.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2022.
300 _aXIII, 264 p. 50 illus., 44 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aNeuromethods,
_x1940-6045 ;
_v185
505 0 _aUsing Lentiviral shRNA Delivery to Knock-Down Proteins in Cultured Neurons and In Vivo -- Synaptosomal Preparation and Its Application in Preclinical Studies -- Assessing the Nanoscale Organization of Excitatory and Inhibitory Synapses using Recombinant Probes to Visualize Endogenous Synaptic Proteins -- Agonist-Induced Functional Analysis and Cell Sorting (aiFACS) to Select and Characterize Interneurons during Brain Development -- A CRISPR/Cas9-Based Toolkit to Test Gain- and Loss-of-Gene Function in Brain Organoids -- Assessment of Adult Mouse Brain NeuroAnatomical Phenotypes using Quantitative and Precision Histology -- Leveraging Microelectrode Array Technology for Phenotyping Stem Cell-Derived Neurodevelopmental Disease Models -- Humanized Chimeric Mouse Models to Study Human Neural Development and Pathogenesis of Brain Diseases -- In Vivo Manipulations to Correct for Behavioral Phenotypes in Cognitive Disorder Mouse Models -- Assessing Dyadic Social Interactions in Rodent Models of Neurodevelopmental Disorders -- Central and Peripheral Explorations by Metabolomics to Study Neurodevelopmental Disorders -- Genetic Engineering of Non-Human Primates Models for Studying Neurodevelopmental Disorders.
520 _aThis volume explores the latest techniques used to study neurodevelopmental diseases (NDD) that range from molecular aspects to integrated research approaches and brain imaging in living rodents. Chapters in this book cover topics such as protocols to deliver shRNA in vitro and in vivo using lentiviral particles to knock-down specific protein expression; experimental procedures to use recombinant fluorescent probes to visualize endogenous proteins at the mammalian synapse; CRISPR/Cas9 toolkit to assess either gain- or loss-of-gene function in brain organoids; detailed protocols to use in vivo manipulations to correct the behavioral phenotypes in cognitive disorder mouse lines; and experimental approaches to genetically engineer macaque models of NDDs and investigate how genetic predisposition may cause neural and functional alterations. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and practical, Translational Research Methods in Neurodevelopmental Disorders is a valuable resource for all researchers who want to learn more about this important and developing field. .
650 0 _aNeurosciences.
650 0 _aMedicine
_xResearch.
650 0 _aBiology
_xResearch.
650 1 4 _aNeuroscience.
650 2 4 _aTranslational Research.
700 1 _aMartin, Stéphane.
_eeditor.
_0(orcid)0000-0001-6771-7645
_1https://orcid.org/0000-0001-6771-7645
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aLaumonnier, Frédéric.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071625682
776 0 8 _iPrinted edition:
_z9781071625705
776 0 8 _iPrinted edition:
_z9781071625712
830 0 _aNeuromethods,
_x1940-6045 ;
_v185
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-2569-9
912 _aZDB-2-PRO
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