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020 _a9781071623992
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024 7 _a10.1007/978-1-0716-2399-2
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082 0 4 _a660.6
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245 1 0 _aYeast Metabolic Engineering
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Valeria Mapelli, Maurizio Bettiga.
250 _a2nd ed. 2022.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2022.
300 _aXI, 295 p. 51 illus., 32 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2513
505 0 _aAn Overview on Selectin Marker Genes for Transformation of Saccharomyces cerevisiae -- High Efficiency Plasmid DNA Transformation in Yeast -- CasPER, A CRISPR/Cas9-Based Method for Directed Evolution in Genomic loci in Saccharomyces cerevisiae -- Fluorescence Activated Cell Sorting as a Tool for Recombinant Strain Screening -- Generation of Arming Yeasts with Active Proteins and Peptides via Cell Surface Display System: Cell Surface Engineering, Bio-Arming Technology -- Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris -- Competent Cell Preparation and Transformation of Pichia pastoris -- CRISPR/Cas9 Tool Kit for Efficient and Targeted Insertion/Deletion Mutagenesis of the Komagataella phaffii (Pichia pastoris) Genome -- Plasmid-Based Gene Knockout Strategy with Subsequent Marker Recycling in Pichia pastoris -- Engineering of Promoters for Gene Expression in Pichia pastoris -- Molecular Tools for Leveraging the Potential of the Acid Tolerant Yeast Zygosaccharomyces bailii as Cell Factory -- Golden Gate Multi-Genes Assembly Method for Yarrowia lipolytica -- A Universal Gene Expression System for Novel Yeast Species -- Dynamic Feeding of Pichia pastoris -- Automated Evolutionary Engineering of Yeasts -- Reconstruction of Genome-Scale Metabolic Model of Hansenula polymorpha using RAVEN.
520 _aThis second edition volume expands on the previous edition with new and updated chapters on the latest developments in the study of yeast within the biotechnology field. The chapters in this book cover topics such as transformation protocols for genetic engineering of Saccaromyces cerevisiae and Komagataella spp.; an overview of selection markers, promoters, and strains used for metabolic engineering of S. cerevisiae, P. pastoris, and Z. bailii; the use of yeast in CRISPR/Cas9 technology; tools to study metabolic pathway in Yarrowia lypolitica; and a discussion on the “universal expression system” that is applied in a broad spectrum of fungal species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Yeast Metabolic Engineering: Methods and Protocols, Second Edition is a valuable resource for researchers and scientists interested in learning more about this important and developing field.
650 0 _aBiotechnology.
650 0 _aMaterials
_xMicroscopy.
650 1 4 _aBiotechnology.
650 2 4 _aMicroscopy.
700 1 _aMapelli, Valeria.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aBettiga, Maurizio.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071623985
776 0 8 _iPrinted edition:
_z9781071624005
776 0 8 _iPrinted edition:
_z9781071624012
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2513
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-2399-2
912 _aZDB-2-PRO
999 _c5649
_d5649