000 04491nam a22004935i 4500
001 978-1-0716-2289-6
003 DE-He213
005 20260610083632.0
007 cr nn 008mamaa
008 220622s2022 xxu| s |||| 0|eng d
020 _a9781071622896
_9978-1-0716-2289-6
024 7 _a10.1007/978-1-0716-2289-6
_2doi
050 4 _aQL921-939
072 7 _aMFC
_2bicssc
072 7 _aSCI056000
_2bisacsh
072 7 _aMFC
_2thema
082 0 4 _a611.8
_223
082 0 4 _a573.833
_223
245 1 4 _aThe Blood-Brain Barrier
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Nicole Stone.
250 _a1st ed. 2022.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2022.
300 _aXI, 357 p. 83 illus., 73 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2492
505 0 _aCells of the Blood-Brain Barrier: An Overview of the Neurovascular Unit in Health and Disease -- In Vitro Models of the Blood-Brain Barrier -- In Vitro Models of the Human Blood-Brain Barrier Based on Human Induced Pluripotent Stem Cells: Opportunities and Challenges -- Induced Pluripotent Stem Cell (iPSC)-Derived Endothelial Cells to Study Bacterial–Brain Endothelial Cell Interactions -- An hiPSC-Derived In Vitro Model of the Blood-Brain Barrier -- A Three-Dimensional Brain-on-Chip Using Human iPSC-Derived GABAergic Neurons and Astrocytes -- An Improved In Vitro Porcine Blood-Brain Barrier Model for Permeability Screening and Functional Studies -- Establishment of an In Vitro Model of Human Blood-Brain Barrier to Study the Impact of Ischaemic Injury -- A Novel Dynamic Human In Vitro Model for Studying the Blood-Brain Barrier -- A Dynamic, In Vitro BBB Model to Study the Effects of Varying Levels of Shear Stress -- Novel, Emerging Chip Models of the BBB and Future Directions -- Integrating Primary Astrocytes in a Microfluidic Model of the Blood-Brain Barrier -- Fabrication of Microtube-Embedded Chip to Mimic Blood-Brain Barrier Capillary Vessels -- BBB-on-a-Chip: Modeling Functional Human BBB by Mimicking 3D Brain Angiogenesis Using Microfluidic Chip -- Cell Interplay Model to Assess the Impact of Glioma Cells on Blood-Brain Barrier Permeability -- An In Vitro Human Blood-Brain Barrier Model to Study Breast Cancer Brain Metastasis -- An In Vivo Mouse Model to Study Blood-Brain Barrier Destabilization in the Chronic Phase of Stroke -- Immunohistochemical Analysis of Tight Junction Proteins -- An In Vitro BBB Model to Study Firm Shear-Stress Resistant Leukocyte Adhesion to Human Brain Endothelial Cells -- An In Vitro Model of the Blood-Brain Barrier (BBB) to Study Alzheimer's Disease: The Role of β-Amyloid and Its Influence on PBMC Infiltration.
520 _aThis detailed volume features techniques to explore the complex interface that separates the systemic circulation from the central nervous system, known as the blood-brain barrier (BBB). Beginning with an introduction to its physiology, the book continues with sections on using pluripotent stem cells in models of the BBB, co-culture, permeability and transwell models, microfluidic and chip models, as well as models to study specific BBB pathologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The Blood-Brain Barrier: Methods and Protocols collects a wide range of methodologies which will aid all researchers in the fascinating world of the blood-brain barrier.
650 0 _aNeuroanatomy.
650 0 _aNeurosciences.
650 1 4 _aNervous System.
650 2 4 _aNeuroscience.
700 1 _aStone, Nicole.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071622889
776 0 8 _iPrinted edition:
_z9781071622902
776 0 8 _iPrinted edition:
_z9781071622919
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2492
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-2289-6
912 _aZDB-2-PRO
999 _c5643
_d5643