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020 _a9781071622131
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024 7 _a10.1007/978-1-0716-2213-1
_2doi
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_2bicssc
072 7 _aMED106000
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082 0 4 _a615.90072
_223
245 1 0 _aHigh-Throughput Screening Assays in Toxicology
_h[electronic resource] /
_cedited by Hao Zhu, Menghang Xia.
250 _a2nd ed. 2022.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2022.
300 _aXII, 200 p. 54 illus., 32 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2474
505 0 _aCell-Based Assays to Identify ERR and ERR/PGC Modulators -- Mitochondrial Membrane Potential Assay -- Cell-Based hERG Inhibition Assay in a High-Throughput Format -- Identifying CAR Modulators Utilizing a Reporter Gene Assay -- Study Liver Cytochrome P450 3A4 Inhibition and Hepatotoxicity Using DMSO-Differentiated HuH-7 Cells -- Acetylcholinesterase Inhibition Assays for High-Throughput Screening -- Cell-Based Assays to Identify Modulators of Nrf2/ARE Pathway -- Cell-Based Imaging Assay for Detection of Phospholipdosis -- GFP-LC3 High-Content Assay for Screening Autophagy Modulators -- Generation of iPSC-Derived Brain Organoids for Drug Testing and Toxicological Evaluation -- Zebrafish Behavioral Assays in Toxicology -- High Throughput Screening Assay Profiling for Large Chemical Databases -- A Quantitative High Throughput Screening Data Analysis Pipeline for Activity Profiling -- CurveP Method for Rendering High Throughput Screening Dose-Response Data into Digital Fingerprints -- Accounting for Artifacts in High Throughput Toxicity Assays -- Automatic Quantitative Structure-Activity Relationship Modeling to Fill Data Gaps in High-Throughput Screening -- Use In Silico and In Vitro Methods to Screen Hepatotoxic Chemicals and CYP450 Enzyme Inhibitors.
520 _aThis second edition volume expands on the previous edition by exploring the latest advancements in high throughput screening (HTS) in toxicity studies by using in vitro, ex vivo, and in vivo models. This volume also covers the application of artificial intelligence (AI) and data science to curate, manage, and use HTS data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, High Throughput Screening Assays in Modern Toxicology, Second Edition is a valuable resource for scientists pursuing chemical toxicology research. This book will aid scientists and researchers in translating new HTS techniques into standardized chemical toxicology assessment tools that can refine, reduce, and replace animal testing. .
650 0 _aToxicology.
650 0 _aBiological assay.
650 1 4 _aToxicology.
650 2 4 _aAssay Systems.
700 1 _aZhu, Hao.
_eeditor.
_0(orcid)0000-0002-3559-6129
_1https://orcid.org/0000-0002-3559-6129
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aXia, Menghang.
_eeditor.
_0(orcid)0000-0001-7285-8469
_1https://orcid.org/0000-0001-7285-8469
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071622124
776 0 8 _iPrinted edition:
_z9781071622148
776 0 8 _iPrinted edition:
_z9781071622155
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2474
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-2213-1
912 _aZDB-2-PRO
999 _c5639
_d5639