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020 _a9781071616017
_9978-1-0716-1601-7
024 7 _a10.1007/978-1-0716-1601-7
_2doi
050 4 _aRC321-580
072 7 _aPSAN
_2bicssc
072 7 _aSCI089000
_2bisacsh
072 7 _aPSAN
_2thema
082 0 4 _a612.8
_223
245 1 0 _aNeural Reprogramming
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Henrik Ahlenius.
250 _a1st ed. 2021.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2021.
300 _aXI, 263 p. 46 illus., 43 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2352
505 0 _aIsolation and Neuronal Reprogramming of Mouse Embryonic Fibroblasts -- Direct In Vitro Reprogramming of Astrocytes into Induced Neurons -- Direct Cell Reprogramming of Mouse Fibroblast into Functional Astrocytes Using Lentiviral Overexpression of the Transcription Factors NFIA, NFIB, and SOX9 -- Direct Reprogramming of Fibroblasts to Astrocytes Using Small Molecules -- Bcl-2-Assisted Reprogramming of Mouse Astrocytes and Human Fibroblasts into Induced Neurons -- Direct Conversion of Human Fibroblasts to Induced Neurons -- Generation of Induced Dopaminergic Neurons from Human Fetal Fibroblasts -- Direct Differentiation of Functional Neurons from Human Pluripotent Stem Cells (hPSCs) -- Generation of Motor Neurons from Human ESCs/iPSCs Using Sendai Virus Vectors -- Transcription Factor Programming of Human Pluripotent Stem Cells to Functionally Mature Astrocytes for Monocultures and Cocultures with Neurons -- Single Transcription Factor-Based Differentiation Allowing Fast and Efficient Oligodendrocyte Generation via SOX10 Overexpression -- Transcriptional Profiling During Neural Conversion -- Functional Assessment of Direct Reprogrammed Neurons In Vitro and In Vivo -- Neurotransmitter Release of Reprogrammed Cells Using Electrochemical Detection Methods -- Combining Cell Fate Reprogramming and Protein Engineering to Study Transcription Factor Functions -- CRISPR/Cas9-Genome Engineering in Human Pluripotent Stem Cells for Modeling of Neurological Disorders -- Derivation of Adult Human Cortical Organotypic Slice Cultures for Co-Culture with Reprogrammed Neuronal Cells.
520 _aThis detailed book brings together a number of state-of-the-art protocols to generate different types of neural cells through the use of reprogramming technologies. Additionally, the volume explores different aspects of functional evaluation and applications of reprogrammed neural cells as well as in silico methods to aid reprogramming efforts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Neural Reprogramming: Methods and Protocols provides ample experimental experience and guidance for anyone, be it experienced researcher or beginner, to generate, validate, and apply reprogrammed neural cells in their research.
650 0 _aNeurosciences.
650 0 _aBiomedical engineering.
650 1 4 _aNeuroscience.
650 2 4 _aBiomedical Engineering and Bioengineering.
700 1 _aAhlenius, Henrik.
_eeditor.
_0(orcid)0000-0001-8958-6148
_1https://orcid.org/0000-0001-8958-6148
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071616000
776 0 8 _iPrinted edition:
_z9781071616024
776 0 8 _iPrinted edition:
_z9781071616031
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2352
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-1601-7
912 _aZDB-2-PRO
999 _c5615
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