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024 7 _a10.1007/978-1-0716-1241-5
_2doi
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082 0 4 _a543.65
_223
245 1 0 _aMass Spectrometry of Glycoproteins
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Arnaud Delobel.
250 _a1st ed. 2021.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2021.
300 _aXIV, 401 p. 140 illus., 126 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2271
505 0 _aGlycosylation of Therapeutic Proteins: A Critical Quality Attribute -- Characterization of Protein Glycoforms at Intact Level by Orbitrap Mass Spectrometry -- Analysis of Intact Glycoproteins by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry -- Fc Glycosylation Characterization of Human Immunoglobulins G using Immunocapture and LC-MS -- Fast Afucosylation Profiling of Glyco-Engineered Antibody Subunits by Middle Up Mass Spectrometry -- Characterization of Glycosylated Proteins at Subunit Level by HILIC/MS -- Analysis of Monoclonal Antibody Glycopeptides by Capillary Electrophoresis – Mass Spectrometry Coupling (CE-MS) -- Enrichment of Intact Glycopeptides using Strong Anion Exchange and Electrostatic Repulsion Hydrophilic Interaction Chromatography -- In-Depth Glycan Characterization of Therapeutic Glycoproteins by Stepwise PGC –SPE and LC-MS/MS -- Site-Specific N-glycosylation Analysis of Recombinant Proteins by LC/MSE -- Mapping O-glycosylation Sites using OpeRATOR and LC-MS -- Site-Specific O-Glycosylation Analysis by Liquid Chromatography/Mass Spectrometry with Electron-Transfer/Higher-Energy Collisional Dissociation -- Profiling of N-Linked Oligosaccharides of a Glycoprotein by UPLC-FLR-ESI-MS after Derivatization with Fluorescent Anthranilamide -- Evaluating N-glycosylation of a Therapeutic Monoclonal Antibody using UHPLC-FLR-MS with RapiFluor-MS Labeling -- Routine Analysis of N-glycans using Liquid Chromatography Coupled to Routine Mass Detection -- Online 2D-LC for Complex N-Glycan Analysis from Biopharmaceuticals -- Profiling, Relative Quantification, and Identification of Sialylated N-Linked Oligosaccharides by UPLC-FLR-ESI/MS after Derivatization with Fluorescent Anthranilamide -- Linkage Analysis of Oligo- and Polysaccharides: A Tutorial -- Use of Exoglycosidases for the Structural Characterization of Glycans -- Determination of Isomeric Glycan Structures by Permethylation and Liquid Chromatography-Mass Spectrometry (LC-MS) -- Optimization of Multiple Glycosidase and Chemical Stabilization Strategies for N-glycan Isomer Detection by Mass Spectrometry Imaging in Formalin-Fixed, Paraffin-Embedded Tissues -- Glycosylation Profiling of Glycoproteins Secreted from Cultured Cells Using Glycan Node Analysis and GC-MS -- Array-Based N-Glycan Profiling of Cells in Culture -- Analysis of Oligomeric and Glycosylated Proteins by Size-Exclusion Chromatography Coupled with Multi-Angle Light Scattering -- Analysis of Glycoproteins by ATR-FTIR Spectroscopy: Comparative Assessment -- Deploying Mass Spectrometric Data Analysis in the Amazon AWS Cloud Computing Environment.
520 _aThis volume presents methods used for the analysis of glycoproteins at different levels—intact, subunit, glycopeptide, and monosaccharide--, and discusses and solves most analytical challenges that a scientist working on glycoproteins may come across. The chapters in this book cover topics such as the role of glycosylation on the properties of therapeutic glycoproteins; different analytical methods to characterize glycosylation, from the intact proteins to the glycan level, for both N-linked and O-linked glycoproteins; mass spectrometry imaging methodology for glycosylation analysis in tissues; approaches to characterizing glycosylation on cultured cells; and the use of cloud computing to deploy mass spectrometry data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Mass Spectrometry of Glycoproteins: Methods and Protocols is a valuable resource for scientists interested in learning more about this developing field.
650 0 _aMass spectroscopy.
650 0 _aChemistry.
650 1 4 _aMass Spectrometry.
650 2 4 _aChemistry.
700 1 _aDelobel, Arnaud.
_eeditor.
_0(orcid)0000-0002-5315-7904
_1https://orcid.org/0000-0002-5315-7904
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071612408
776 0 8 _iPrinted edition:
_z9781071612422
776 0 8 _iPrinted edition:
_z9781071612439
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2271
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-1241-5
912 _aZDB-2-PRO
999 _c5607
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