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020 _a9781071609828
_9978-1-0716-0982-8
024 7 _a10.1007/978-1-0716-0982-8
_2doi
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_2bicssc
072 7 _aSCI045000
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_223
245 1 0 _aListeria Monocytogenes
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Edward M. Fox, Hélène Bierne, Beatrix Stessl.
250 _a2nd ed. 2021.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2021.
300 _aXI, 260 p. 54 illus., 30 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2220
505 0 _aTraditional Methods of Analysis for Listeria monocytogenes -- MALDI-ToF MS: A Rapid Methodology for Identifying and Subtyping Listeria monocytogenes -- Sample Preparation for qPCR Detection of Listeria from Food -- qPCR Validation on the Basis of the Listeria monocytogenes prfA Assay -- Serotype Assignment by Sero-Agglutination, ELISA, and PCR -- Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Listeria monocytogenes -- Multi-Locus Sequence Typing (MLST) and Whole Genome Sequencing (WGS) of Listeria monocytogenes and Listeria innocua -- High-Throughput Characterization of Listeria monocytogenes Using the OmniLog Phenotypic Microarray -- High-Throughput Screening of Biofilm Formation of Listeria monocytogenes on Stainless Steel Coupons Using a 96-Well Plate Format -- Confocal Laser Microscopy Analysis of Listeria monocytogenes Biofilms and Spatially Organized Communities -- Extraction and Preparation of Listeria monocytogenes Subproteomes for Mass Spectrometry Analysis -- Extraction and Analysis of Plasmid DNA from Listeria monocytogenes -- Generation of Nonpolar Deletion Mutants in Listeria monocytogenes Using the “SOEing” Method -- Mutant Construction and Integration Vector-Mediated Genetic Complementation in Listeria monocytogenes -- Internalization Assays for Listeria monocytogenes -- Microscopy of Intracellular Listeria monocytogenes in Epithelial Cells -- Control of Listeria monocytogenes Biofilms in a Simulated Food Processing Environment -- Sampling the Food Processing Environment: Taking up the Cudgel for Preventive Quality Management in Food Processing (FP) -- Isolation and Evaluation of Anti-Listeria Lactococcus lactis from Vegetal Sources.
520 _aThis updated book explores a wide repertoire of tools and approaches that have been created, modified, and applied to the study of L. monocytogenes, forming the basis of our understanding of the bacterium today. Many of these key experimental techniques are gathered together herein. The volume presents aspects such as clinical disease and host-pathogen interactions, as well as the study of biofilms which present a significant challenge for control of the organism in the food processing environment. The topics covered in this edition also include sampling in order to isolate Listeria, methods for their identification and characterization, methods for gene manipulation, and methods for control of the organism. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshootingand avoiding known pitfalls. Authoritative and up-to-date, Listeria monocytogenes: Methods and Protocols, Second Edition aims to contribute toward the harmonization of methods used to study this important bacterium, and to be of particular interest to Listeria research both in relation to food association and control as well as clinical microbiology.
650 0 _aBacteria.
650 0 _aFood science.
650 0 _aFood
_xMicrobiology.
650 1 4 _aBacteria.
650 2 4 _aFood Science.
650 2 4 _aFood Microbiology.
700 1 _aFox, Edward M.
_eeditor.
_0(orcid)0000-0001-5850-7558
_1https://orcid.org/0000-0001-5850-7558
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aBierne, Hélène.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aStessl, Beatrix.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071609811
776 0 8 _iPrinted edition:
_z9781071609835
776 0 8 _iPrinted edition:
_z9781071609842
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2220
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-0982-8
912 _aZDB-2-PRO
999 _c5589
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