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001 978-1-59259-207-4
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020 _a9781592592074
_9978-1-59259-207-4
024 7 _a10.1385/1592592074
_2doi
050 4 _aRC321-580
072 7 _aPSAN
_2bicssc
072 7 _aSCI089000
_2bisacsh
072 7 _aPSAN
_2thema
082 0 4 _a612.8
_223
245 1 0 _aProtocols for Neural Cell Culture
_h[electronic resource] /
_cedited by Sergey Fedoroff, Arleen Richardson.
250 _a3rd ed. 2001.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2001.
300 _aXXII, 262 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aSpringer Protocols Handbooks,
_x1949-2456
505 0 _aIn Vitro Assays for Axonal Growth and Targeting -- Interface Organotypic Hippocampal Slice Cultures -- Slice Cultures for Study of Microglia -- Microexplant Cultures of the Cerebellum -- Construction and Use of Compartmented Cultures for Studies of Cell Biology of Neurons -- Chick Spinal Somatic Motoneurons in Culture -- Primary Cultures of Sympathetic Ganglia -- Primary Cell Cultures for the Study of Myelination -- Preparation of Astrocyte, Oligodendrocyte, and Microglia Cultures from Primary Rat Cerebral Cultures -- Culture of Glial Cells from Human Brain Biopsies -- Cultures of Astroglia and Microglia from Primary Cultures of Mouse Neopallium -- Cultures of Oligodendroblasts from Primary Cultures of Rat Neopallium -- Olfactory Ensheathing Cell Cultures -- Cultures of Stem Cells of the Central Nervous System -- Aggregating Neural Cell Cultures -- Neural Cell Lines -- Virus Vectors for Gene Therapy of the Nervous System -- Preparation of Substrata for In Vitro Culture of Neurons -- Serum-Free Media forNeural Cell Cultures -- Development of Multiple-Well Plate Biological Assays -- Neural Cell Counting -- Assay for Neuronal Cell Migration -- Colony Cultures -- Hybridoma Cultures for Production of Antibodies -- Elimination of Cell Types from Mixed Neural Cell Cultures -- Quantification of Cells in Culture -- Tips for Tissue Culture.
520 _aIn Protocols for Neural Cell Culture, Third Ed., Sergey Fedoroff and Arleen Richardson extensively revise, update, and expand their best-selling and highly praised collection of readily reproducible neural tissue culture protocols. This 3rd edition adds 11 chapters describing important new procedures for the isolation, growth, and characterization of neural stem cells and for the manipulation of glial progenitor cells, as well as essential procedures for hippocampal and microglial slice cultures and transfection of neurons in culture with adenovirus. It includes key techniques for the preparation of substrata, the use of serum-free media, maintaining hybridomas, and the production and purification of monoclonal antibodies. For scientists not trained in neuroanatomy, but faced with dissecting the brain and spinal cord, most chapters in the 3rd edition provide fully detailed dissection procedures. Protocols for Neural Cell Culture, Third Ed. is a richly augmented updating of the tried and tested laboratory procedures that have made earlier editions an indispensable reference and guide to neural cell culture. Its unique wealth of practical detail on a wide range of tissue culture systems having many applications ensure that this new edition will remain an essential resource for all investigators using cell culture methodology in studying the brain and its disorders.
532 8 _aAccessibility summary: This PDF is not accessible. It is based on scanned pages and does not support features such as screen reader compatibility or descriptions for non-text content (e.g., images and graphs). However, it likely supports searchable and selectable text based on OCR (Optical Character Recognition). Users with accessibility needs may not be able to use this content effectively. Please contact us at through this accessibility request webform if you require assistance or an alternative format.
532 8 _aInaccessible, or known limited accessibility
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aNeurosciences.
650 1 4 _aNeuroscience.
700 1 _aFedoroff, Sergey.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aRichardson, Arleen.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896039025
776 0 8 _iPrinted edition:
_z9781489941992
830 0 _aSpringer Protocols Handbooks,
_x1949-2456
856 4 0 _uhttps://doi.org/10.1385/1592592074
912 _aZDB-2-PRO
999 _c5573
_d5573