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020 _a9781071630242
_9978-1-0716-3024-2
024 7 _a10.1007/978-1-0716-3024-2
_2doi
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072 7 _aPST
_2bicssc
072 7 _aSCI011000
_2bisacsh
072 7 _aPST
_2thema
082 0 4 _a580
_223
245 1 0 _aPlant Genotyping
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Yuri Shavrukov.
250 _a1st ed. 2023.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2023.
300 _aXVI, 469 p. 92 illus.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2638
505 0 _aGenotyping by Sequencing (GBS) for Genome-Wide SNP Identification in Plants -- Genotyping by Multiplexed Sequencing (GMS) Using SNP Markers -- Computational Protocol for DNA Methylation Profiling in Plants Using Restriction Enzyme-Based Genome Reduction -- Double Digest Restriction-Site Associated DNA Sequencing (ddRADseq) Technology -- Whole Genome Wide SSR Markers Identification Based on ddRAD-Seq Data -- High-Throughput Association Mapping in Brassica napus L.: Methods and Applications -- Polyploid SNP Genotyping Using the MassARRAY System -- qPCR Genotyping of Polyploid Species -- Genome-Wide Association Studies (GWAS) -- Transcriptomic Approach for Global Distribution of SNP/Indel and Plant Genotyping -- Specific-Locus Amplified Fragment Sequencing (SLAF-Seq) -- Modifications of Kompetitive Allele-Specific PCR (KASP) Genotyping for Detection of Rare Alleles -- Amplifluor-Based SNP Genotyping -- SNP Genotyping with Amplifluor-Like Method -- Semi-Thermal Asymmetric Reverse PCR (STARP) Genotyping -- Modified Allele-Specific qPCR (ASQ) Genotyping -- Allele-Specific Mutation Genotyping with Mismatches in Primer Design -- PCR Allele Competitive Extension (PACE) -- Molecular Beacon Probe (MBP)-Based Real Time PCR -- Molecular Beacons – Loop-Mediated Amplification (MB-LAMP) -- TaqMan Probes for Plant Species Identification and Quantification in Food and Feed Traceability -- Tetra-Primer Amplification Refractory Mutation System (T-ARMS) -- Penta-Primer Amplification Refractory Mutation System (PARMS) with Direct PCR-Based SNP Marker-Assisted Selection (D-MAS) -- High-Resolution Melting (HRM) Genotyping -- Modified High-Resolution Melting (HRM) Marker Systems Increasing Discriminability Between Homozygous Alleles -- A New SNP Genotyping Technology by Target SNP-Seq -- Derived Polymorphic Amplified Cleaved Sequence (dPACS) Assay -- Tubulin-Based Polymorphism (TBP) in Plant Genotyping -- Multiplexed ISSR Genotyping by Sequencing (MIG-Seq) -- Application of SolCAP Genotypingin Potato (Solanum tuberosum l.) Association Mapping -- Fluorescence In Situ Hybridization (FISH) for Genotyping of Triticeae Tribe Species and Hybrids -- Innovative Advances in Plant Genotyping.
520 _aThis thorough volume presents a wide range of existing methods, from the very popular to the more exotic, in the area of plant genotyping. Many methods of plant genotyping were initially developed for medical research, but all genotyping methods, if they are to be successful, should be suitable for application across the full range of studies within plant biology, as seen in this collection. Plant genotyping methods herein are based on a variety of assessments, including DNA microarray, with its hundreds of thousands of simultaneous reactions, or separate individual studies of DNA sequencing and fragment analysis, PCR and qPCR, allele-specific molecular probes and primers, digestion with restriction endonucleases, microscopy and many others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, andtips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Genotyping: Methods and Protocols offers researchers the opportunity to update their knowledge and choose the most suitable method of plant genotyping for their chosen application.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aBotany.
650 0 _aPlant genetics.
650 0 _aPhenology.
650 1 4 _aPlant Science.
650 2 4 _aPlant Genetics.
650 2 4 _aPhenology.
700 1 _aShavrukov, Yuri.
_eeditor.
_0(orcid)0000-0002-2442-0398
_1https://orcid.org/0000-0002-2442-0398
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071630235
776 0 8 _iPrinted edition:
_z9781071630259
776 0 8 _iPrinted edition:
_z9781071630266
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2638
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-3024-2
912 _aZDB-2-PRO
999 _c5565
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