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020 _a9781071623251
_9978-1-0716-2325-1
024 7 _a10.1007/978-1-0716-2325-1
_2doi
050 4 _aQD96.M3
072 7 _aPNFS
_2bicssc
072 7 _aSCI013010
_2bisacsh
072 7 _aPNFS
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082 0 4 _a543.65
_223
245 1 0 _aProteoform Identification
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Liangliang Sun, Xiaowen Liu.
250 _a1st ed. 2022.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2022.
300 _aX, 240 p. 82 illus., 74 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2500
505 0 _aProteoforms and Proteoform Families: Past, Present, and Future -- Membrane Ultrafiltration-Based Sample Preparation Method and Sheath-Flow CZE-MS/MS for Top-Down Proteomics -- Size Exclusion Chromatography Strategies and MASH Explorer for Large Proteoform Characterization -- RPLC-RPLC-MS/MS for Proteoform Identification -- Monolithic Materials-Based RPLC-MS for Proteoform Separation and Identification -- Capillary Isoelectric Focusing: Mass Spectrometry Method for the Separation and Online Characterization of Monoclonal Antibody Charge Variants at Intact and Subunit Level -- Proteoform Analysis and Construction of Proteoform Families in Proteoform Suite -- Top-Down Mass Spectrometry Data Analysis Using TopPIC Suite -- Accurate Proteoform Identification and Quantitation Using pTop 2.0 -- Proteoform Identification and Quantification using Intact Protein Database Search Engine ProteinGoggle -- Mass Deconvolution of Top-Down Mass Spectrometry Datasets by FLASHDeconv -- Deconvolving Native and Intact Protein Mass Spectra with UniDec -- Discovery of Unknown Post-Translational Modifications by Top-Down Mass Spectrometry -- Determining Copper and Zinc Content in Superoxide Dismutase using Electron Capture Dissociation under Native Spray Conditions -- Surface-Induced Dissociation for Protein Complex Characterization.
520 _aThis volume discusses the latest mass spectrometry (MS)-based technologies for proteoform identification, characterization, and quantification. Some of the topics covered in this book include sample preparation, proteoform separation, proteoform gas-phase fragmentation, and bioinformatics tools for MS data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Proteoform Identification: Methods and Protocols is a valuable resource for researchers in both academia and the biopharmaceutical industry who are interested in proteoform analysis using MS. .
650 0 _aMass spectroscopy.
650 1 4 _aMass Spectrometry.
700 1 _aSun, Liangliang.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aLiu, Xiaowen.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071623244
776 0 8 _iPrinted edition:
_z9781071623268
776 0 8 _iPrinted edition:
_z9781071623275
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2500
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-2325-1
912 _aZDB-2-PRO
999 _c5557
_d5557