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020 _a9781071630525
_9978-1-0716-3052-5
024 7 _a10.1007/978-1-0716-3052-5
_2doi
050 4 _aQH573-671
072 7 _aPSF
_2bicssc
072 7 _aSCI017000
_2bisacsh
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_2thema
082 0 4 _a571.6
_223
245 1 0 _aCell Viability Assays
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Oliver Friedrich, Daniel F. Gilbert.
250 _a2nd ed. 2023.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2023.
300 _aXVII, 502 p. 111 illus., 110 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2644
505 0 _aAssaying Mitochondrial Respiration as an Indicator of Cellular Metabolism and Fitness -- The MTT Assay: A Method for Error Minimization and Interpretation in Measuring Cytotoxicity and Estimating Cell Viability -- Assaying Proliferation Characteristics of Cells Cultured Under Static Versus Periodic Conditions -- Network Reconstruction as a Novel High-Level Marker of Functional Neuronal Viability -- Assaying Mitochondrial Function by Multi-Parametric Flow Cytometry -- High-Efficiency Single Cell Electrical Impedance Spectroscopy -- Cell Viability Multiplexing: Quantification of Cellular Viability by Barcode Flow Cytometry and Computational Analysis -- Average Rheological Quantities of Cells in Monolayers -- Assaying Spontaneous Network Activity and Cellular Viability Using Multi-Well Microelectrode Arrays -- Quantitative Live-Cell Ca2+ Imaging During Isotropic Cell Stretch -- Assessment of Cell Viability in Electrically Excitable Muscle Cells through Intact Twitch Stimulation.-Evaluating Cellular Viability by iTRAQ Proteomic Profiling -- Quantifying pH in Malaria Using pHluorin and Flow Cytometry -- Cell Membrane State, Permeability, and Elasticity Assessment for Single Cells and Cell Ensembles -- Neutral Red Uptake Assay to Assess Cytotoxicity In Vitro -- Digital Holographic Microscopy to Assess Cell Behavior -- Second Harmonic Generation Morphometry of Muscle Cytoarchitecture in Living Cells -- Optimization of Cell Viability Assays for Drug Sensitivity Screens -- Cellasys #8: A Microphysiometric Assay for Real-Time Cell Analysis within 24 Hours -- Live Cell Adhesion, Migration, and Invasion Assays -- Cell Viability and Immunogenic Function of T Cells Loaded with Nanoparticles for Spatial Guidance in Magnetic Fields -- Transwell In Vitro Cell Migration and Invasion Assays -- Live Cell Calcium Imaging of Non-Adherent Cells -- FUCCI Reporter Gene-Based Cell Cycle Analysis -- Cell Viability Assays for 3D Cellular Constructs -- Toxicological Analysis by Assessment of Vascularization and Cell Viability Using the Chicken's Chorioallantoic Membrane (CAM Assay) -- Assessment of Tissue Viability by Functional Imaging of Membrane Potential -- Multiplexed Viability Assays for High-Throughput Screening of Spheroids of Multiple Sizes -- Tracking Gut Motility in Organ and Cultures -- Optimized Method of 3D Scaffold Seeding, Cell Cultivation, and Monitoring Cell Status for Bone Tissue Engineering.
520 _aThis updated edition explores assessing cell viability as a measure for cell fitness under conditions of physiological and patho-physiological stress as well as challenging conditions to cellular and tissue homeostasis, and accounts for the ongoing 2D-to-3D development with topics and assays that target cell viability, mobility, and functionality of tissues and organs, natural or bioartificial, in 3D. The book’s contents span a wide range of viability and functionality assays, from impedance spectroscopy to chemiluminescence, fluorescence and label-free optical detection methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Cell Viability Assays: Methods and Protocols, Second Edition serves as a valuable resource to the growing community in bioinspired life sciences, biomedical sciences, and biotechnology by providing more standardized protocols to probe the “wellbeing” of cells in various environments.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aCytology.
650 0 _aBiological assay.
650 1 4 _aCell Biology.
650 2 4 _aAssay Systems.
700 1 _aFriedrich, Oliver.
_eeditor.
_0(orcid)0000-0003-2238-2049
_1https://orcid.org/0000-0003-2238-2049
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aGilbert, Daniel F.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071630518
776 0 8 _iPrinted edition:
_z9781071630532
776 0 8 _iPrinted edition:
_z9781071630549
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2644
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-3052-5
912 _aZDB-2-PRO
999 _c5465
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