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024 7 _a10.1007/978-1-0716-2659-7
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245 1 0 _aSalamanders
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Ashley W. Seifert, Joshua D. Currie.
250 _a1st ed. 2023.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2023.
300 _aXIV, 483 p. 103 illus., 89 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2562
505 0 _aSalamanders as key models for development and regeneration research -- Establishing a new research axolotl colony -- Health monitoring for laboratory salamanders -- Husbandry, captive breeding and field survey of Chinese giant salamander (Andrias davidianus) -- Whole mount in situ hybridization (WISH) for salamander embryos and larvae -- Hybridization chain reaction fluorescence in situ hybridization (HCR-FISH) in Ambystoma mexicanum tissue -- Ethyl cinnamate-based tissue clearing strategies -- Induction and characterization of cellular senescence in salamanders -- Methods for studying appendicular skeletal biology in axolotls -- Fluorescence in situ hybridization of DNA-probes on mitotic chromosomes of the Mexican axolotl -- The use of small molecules to dissect developmental and regenerative processes in axolotls -- COMET Assay for detection of DNA damage during axolotl tail regeneration -- In vivo and Ex vivo View of Newt Lens Regeneration -- Bead implantation and delivery of exogenous growth factors -- The Accessory Limb Model regenerative assay and its derivatives -- Embryonic Tissue and Blastema Transplantations -- Retinoic acid-induced limb duplications -- Isolation and characterization of peritoneal macrophages from salamanders -- Navigation and use of custom tracks within the axolotl genome browser -- Chromosome Conformation Capture for large genomes -- Axolotl transgenesis via injection of I-SceI meganuclease or Tol2 transposon system -- A practical guide for CRISPR-Cas9-induced mutations in axolotls -- Applying a knock-in strategy to create reporter tagged knockout alleles in axolotls -- Baculovirus production and infection in axolotls -- Cell dissociation techniques in salamanders -- Axolotl metabolism: measuring metabolic energy -- Artificial insemination in axolotls -- Screening salamanders for symbionts -- Assessing leukocyte profiles of salamanders and other amphibians: a herpetologists’ guide -- Best practices to promote data utility and recycling by the salamander community -- Now that we got there, what's next?. .
520 _aThis volume provides a comprehensive reference for researchers aiming to bring new techniques and approaches to their scientific research using urodeles. Chapters are authored by leaders in the field and meant to guide readers through laboratory colony husbandry, traditional molecular techniques, experimental manipulation and surgeries, bioinformatics and genomics, transgenics and lineage-tracing, and physiological and organismal techniques. In addition to laboratory methods, this volume highlights techniques developed for field studies and work with wild-caught animals. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and state-of-the-art, Methods in Salamander Research aims to be a practical guide for researchers interested in deploying new methodologies in their lab or in the field. .
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aBiological models.
650 0 _aMolecular biology.
650 1 4 _aBiological Models.
650 2 4 _aMolecular Biology.
700 1 _aSeifert, Ashley W.
_eeditor.
_0(orcid)0000-0001-6576-3664
_1https://orcid.org/0000-0001-6576-3664
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_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aCurrie, Joshua D.
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_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071626580
776 0 8 _iPrinted edition:
_z9781071626603
776 0 8 _iPrinted edition:
_z9781071626610
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2562
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-2659-7
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