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020 _a9781071626634
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024 7 _a10.1007/978-1-0716-2663-4
_2doi
050 4 _aQD415-436
072 7 _aPSB
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072 7 _aSCI007000
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072 7 _aPSB
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082 0 4 _a572
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245 1 0 _aPhase-Separated Biomolecular Condensates
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Huan-Xiang Zhou, Jan-Hendrik Spille, Priya R. Banerjee.
250 _a1st ed. 2023.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2023.
300 _aXIV, 449 p. 1 illus.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2563
505 0 _aCalculating Binodals and Interfacial Tension of Phase-Separated Condensates from Molecular Simulations, with Finite-Size Corrections -- Field-Theoretic Simulation Method to Study the Liquid-Liquid Phase Separation of Polymers -- Numerical Techniques for Applications of Analytical Theories to Sequence-Dependent Phase Separations of Intrinsically Disordered Proteins -- An Introduction to the Stickers-and-Spacers Framework as Applied to Biomolecular Condensates -- Multi-Scale Modeling of Protein-RNA Condensation In and Out of Equilibrium -- Fluorescence Lifetime Imaging Microscopy of Biomolecular Condensates -- Single-Molecule Fluorescence Methods to Study Protein-RNA Interactions Underlying Biomolecular Condensates -- Fluorescence Correlation Spectroscopy and Phase Separation -- Measurement of Protein and Nucleic Acid Diffusion Coefficients within Biomolecular Condensates Using In-Droplet Fluorescence Correlation Spectroscopy -- Single-Molecule Imaging of the Phase Separation-Modulated DNA Compaction to Study Transcriptional Repression -- Phase Separation-Based Biochemical Assays for Biomolecular Interactions -- Determining Thermodynamic and Material Properties of Biomolecular Condensates by Confocal Microscopy and Optical Tweezers -- A High-Throughput Method to Profile Protein Liquid-Liquid Phase Separation -- Phase Separation of Rubisco by the Folded SSUL Domains of CcmM in Beta-Carboxysome Biogenesis -- Cryo-Electron Tomography of Reconstituted Biomolecular Condensate -- Sedimentation Assays to Assess the Impact of Post-Translational Modifications on Phase Separation of RNA-Binding Proteins In Vitro and In Cells -- Synthetic Organelles for Multiple mRNA Selective Genetic Code Expansion in Eukaryotes -- Single Molecule Tracking of RNA Polymerase In and Out of Condensates in Live Bacterial Cells -- An Optogenetic Toolkit for the Control of Phase Separation in Living Cells -- Assessing the Phase Separation Propensity of Proteins in Living Cells Through Optodroplet Formation -- Mass Balance Imaging: A Phase Portrait Analysis for Characterizing Growth Kinetics of Biomolecular Condensates -- Characterizing Properties of Biomolecular Condensates Below the Diffraction Limit In Vivo.
520 _aThis volume provides readers with a broad collection of theoretical, computational, and experimental methods to quantitatively study the properties of phase-separate biomolecular condensates in diverse systems. The chapters in this book cover topics such as theoretical and computational methods; methods for in vitro characterization of biomolecular condensates; and techniques that enable in-cell characterization of biomolecular condensates. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Phase-Separated Biomolecular Condensates: Methods and Protocols is a valuable resource that helps researchers learn and use established methods to study both biophysical properties and biologicalfunctions of biomolecular condensates. .
650 0 _aBiochemistry.
650 0 _aBioinformatics.
650 1 4 _aBiochemistry.
650 2 4 _aComputational and Systems Biology.
700 1 _aZhou, Huan-Xiang.
_eeditor.
_0(orcid)0000-0001-9020-0302
_1https://orcid.org/0000-0001-9020-0302
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_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aSpille, Jan-Hendrik.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aBanerjee, Priya R.
_eeditor.
_0(orcid)0000-0002-6169-1461
_1https://orcid.org/0000-0002-6169-1461
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_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071626627
776 0 8 _iPrinted edition:
_z9781071626641
776 0 8 _iPrinted edition:
_z9781071626658
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2563
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-2663-4
912 _aZDB-2-PRO
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