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020 _a9781493986330
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024 7 _a10.1007/978-1-4939-8633-0
_2doi
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072 7 _aPSAK
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082 0 4 _a581.35
_223
245 1 0 _aFunctional Genomics in Medicago truncatula
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Luis A. Cañas, José Pío Beltrán.
250 _a1st ed. 2018.
264 1 _aNew York, NY :
_bSpringer New York :
_bImprint: Humana,
_c2018.
300 _aXI, 343 p. 45 illus., 35 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1822
505 0 _aGrain and forage legumes: nutritional value and agriculture sustainability -- Model legumes: functional genomics tools in Medicago truncatula -- The Medicago truncatula genome: genomic data availability -- Physical mutagenesis in Medicago truncatula using Fast Neutron Bombardment (FNB) for symbiosis and developmental biology studies -- Targeting Induced Local Lesions IN Genomes (TILLING) in Medicago truncatula -- T-DNA insertional mutagenesis and activation tagging in Medicago truncatula -- Tnt1 Insertional Mutagenesis in Medicago truncatula -- Transient post-transcriptional gene silencing in Medicago truncatula: Virus-Induced Gene Silencing (VIGS) -- Stable inactivation of microRNAs in Medicago truncatula roots -- Non-isotopic RNA in situ hybridization for functional analyses in Medicago truncatula -- In situ hybridization method for localization of mRNA molecules in Medicago tissue sections -- Editing the Medicago truncatula genome: targeted mutagenesis using the CRISPR-Cas9 reagent -- Functional genomics and seed development in Medicago truncatula: an overview -- Functional genomics and genetic control of compound leaf development in Medicago truncatula: an overview -- Root development in Medicago truncatula: lessons from genetics to functional genomics -- The multiple faces of the Medicago-Sinorhizobium symbiosis -- Functional genomics and flowering time in Medicago truncatula: an overview -- Functional genomics and genetic control of flower and fruit development in Medicago truncatula: an overview -- Towards unravelling the genetic determination of the acquisition of salt and osmotic stress tolerance through in vitro selection in Medicago truncatula -- Functional genomics in the study of metabolic pathways in Medicago truncatula: an overview.
520 _aThis volume discusses popular methods to achieve different types of mutagenesis and forward/reverse genetics in Medicago truncatula. Several studies on genetic control of developmental and metabolic processes in this model legume are also described. The chapters in this book cover topics such as Targeting Induced Local Lesions IN Genomes (TILLING), Fast Neutron Bombardment (FNB), Tnt1 insertional mutagenesis, Virus-Induced Gene Silencing (VIGS), stable inactivation of microRNAs in roots, gene editing by CRISPR-Cas9, etc. This book also contains reviews on the specific use of these techniques in functional studies on the genetic control of seed, leaf, root, nodule, floral and fruit development in M. truncatula. Written for the highly successful Methods in Molecular Biology series format, chapters contain the kind of detailed description and implementation advice needed to promote success in the lab. Cutting-edge and thorough, Functional Genomics in Medicago truncatula: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing field.
650 0 _aPlant genetics.
650 1 4 _aPlant Genetics.
700 1 _aCañas, Luis A.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aBeltrán, José Pío.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781493986323
776 0 8 _iPrinted edition:
_z9781493986347
776 0 8 _iPrinted edition:
_z9781493993482
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1822
856 4 0 _uhttps://doi.org/10.1007/978-1-4939-8633-0
912 _aZDB-2-PRO
999 _c5388
_d5388