000 03903nam a22004575i 4500
001 978-1-59259-572-3
003 DE-He213
005 20260610083628.0
007 cr nn 008mamaa
008 100301s1998 xxu| s |||| 0|eng d
020 _a9781592595723
_9978-1-59259-572-3
024 7 _a10.1385/0896034879
_2doi
050 4 _aQD415-436
072 7 _aPSB
_2bicssc
072 7 _aSCI007000
_2bisacsh
072 7 _aPSB
_2thema
082 0 4 _a572
_223
245 1 0 _aProtein Targeting Protocols
_h[electronic resource] /
_cedited by Roger A. Clegg.
250 _a1st ed. 1998.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c1998.
300 _aXII, 331 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v88
505 0 _aSurface Plasmon Resonance -- Microcalorimetry of Protein-Protein Interactions -- Partitioning of Proteins in Triton X-114 -- Co-Immunoprecipitation -- Overlay, Ligand Blotting, and Band-Shift Techniques to Study Kinase Anchoring -- Size-Exclusion Chromatography -- Overlay and Bead Assay -- Use of GST-Fusion and Related Constructs for the Identification of Interacting Proteins -- Molecular Genetic Approaches I -- Molecular Genetic Approaches II -- Molecular Genetic Approaches III -- Molecular Genetic Approaches IV -- Use of Synthetic Peptides in the Dissection of Protein-Targeting Interactions -- SH2 and SH3 Domains -- PH Domain of Serine-Threonine Protein Kinase B (RAC-PKB) -- Membrane Targeting via Protein N-Myristoylation -- Membrane Targeting via Protein Palmitoylation -- Agonist-Mediated Turnover of G-Protein ?-Subunit Palmitoyl Groups -- Membrane Targeting via Protein Prenylation -- Nuclear Protein Import in a Permeabilized Cell Assay -- Clathrin-Coated Vesicles and Targeting -- Analysis of the Sorting of Secretory Proteins to the Regulated Secretory Pathway.
520 _aIn Protein Targeting Protocols, master experimentalists describe, largely from the perspective of intracellular signaling, their best time-tested methods for detecting and analyzing the proteins and protein motifs involved in cellular targeting interactions. The techniques range from basic methods that reveal the existence of targeting interactions and characterize physicochemical properties to those for analyzing the more structurally elaborate systems involved in the movement of molecules between cellular compartments, with examples taken from nuclear transport, clathrin-coated vesicle traffic, and secretory sorting. The themes of targeting domains, particularly the SH-2, SH-3, and PH domains, and the principal targeting of lipid modifications-myristoylation, reversible palmitoylation, prenylation-are fully explored. Protein Targeting Protocols offers a valuable collection of both basic and state-of-the-art techniques suitable for use in all areas of molecular cell biology by researchers interested in defining and characterizing the wide variety of protein interactions underlying targeting mechanisms. Readers will find it serves well as a firm methodological foundation for laboratories newly entering this field and as an indispensable reference work for those already well-established in it.
650 0 _aBiochemistry.
650 1 4 _aBiochemistry.
700 1 _aClegg, Roger A.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896034501
776 0 8 _iPrinted edition:
_z9780896034877
776 0 8 _iPrinted edition:
_z9781489942999
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v88
856 4 0 _uhttps://doi.org/10.1385/0896034879
912 _aZDB-2-PRO
999 _c5380
_d5380