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001 978-3-662-53118-1
003 DE-He213
005 20260610083627.0
007 cr nn 008mamaa
008 161130s2017 gw | s |||| 0|eng d
020 _a9783662531181
_9978-3-662-53118-1
024 7 _a10.1007/978-3-662-53118-1
_2doi
050 4 _aQR
072 7 _aPSG
_2bicssc
072 7 _aSCI045000
_2bisacsh
072 7 _aPSG
_2thema
082 0 4 _a579
_223
245 1 0 _aHydrocarbon and Lipid Microbiology Protocols
_h[electronic resource] :
_bField Studies /
_cedited by Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales.
250 _a1st ed. 2017.
264 1 _aBerlin, Heidelberg :
_bSpringer Berlin Heidelberg :
_bImprint: Springer,
_c2017.
300 _aX, 362 p. 70 illus., 50 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aSpringer Protocols Handbooks,
_x1949-2456
505 0 _aIntroduction -- Marine Systems -- Coastline/Intertidal Systems -- Terrestrial Systems -- Petroleum Reservoirs -- Aquifers/Groundwater -- Freshwater Lakes, Rivers -- Investigating the Biodegradation of Volatile Hydrocarbons in Diverse Environments.
520 _aThis Volume describes methods for investigating microbes in their natural environment and how to obtain representative samples and preserve them for subsequent analyses. Chapters are arranged according to the environments under investigation, which include: oil reservoirs, fracking fluids, aquifers, coal beds, oil sands and their tailing ponds, lakes, rivers, leaves, polar seas and ice, the sea-surface microlayer, mud flats, microbialites, and deep-sea fauna. A variety of downstream analytical procedures are described, including: nucleic-acid extraction and preparation for high-throughput sequencing, fluorescence in-situ hybridisation, and cultivation of aerobic and anaerobic hydrocarbon-degrading microbes. Though most chapters focus on hydrocarbon-rich environments, many of the approaches used are generic, and as such will be of value to researchers embarking on studies of microbes and their processes in the field. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
650 0 _aMicrobiology.
650 0 _aBiotechnology.
650 1 4 _aMicrobiology.
650 2 4 _aChemical Bioengineering.
700 1 _aMcGenity, Terry J.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aTimmis, Kenneth N.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aNogales, Balbina.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9783662531167
776 0 8 _iPrinted edition:
_z9783662531174
776 0 8 _iPrinted edition:
_z9783662571156
830 0 _aSpringer Protocols Handbooks,
_x1949-2456
856 4 0 _uhttps://doi.org/10.1007/978-3-662-53118-1
912 _aZDB-2-PRO
999 _c5378
_d5378