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001 978-1-59259-178-7
003 DE-He213
005 20260610083627.0
007 cr nn 008mamaa
008 100301s2002 xxu| s |||| 0|eng d
020 _a9781592591787
_9978-1-59259-178-7
024 7 _a10.1385/1592591787
_2doi
050 4 _aQH573-671
072 7 _aPSF
_2bicssc
072 7 _aSCI017000
_2bisacsh
072 7 _aPSF
_2thema
082 0 4 _a571.6
_223
245 1 0 _aTransgenesis Techniques
_h[electronic resource] :
_bPrinciples and Protocols /
_cedited by Alan R. Clarke.
250 _a2nd ed. 2002.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2002.
300 _aX, 351 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v180
505 0 _aTopical Reviews in Transgenesis -- Biomedical and Agricultural Applications of Animal Transgenesis -- Transgenesis in Invertebrate and Lower Vertebrate Species -- Gene Transfer in Drosophila -- Transgenesis in the Mouse: Oocyte Injection -- Oocyte Injection in the Mouse -- Alternative Routes to the Germline -- Adenoviral Infection -- Retroviral Infection -- Transgenesis in the Mouse: The ES Cell Route -- In Vitro Isolation of Murine Embryonic Stem Cells -- Production of Chimeras Derived from Murine Embryonic Stem Cells -- Gene-Targeting Strategies -- Cre/loxP Recombination System and Gene Targeting -- Cryopreservation of Mouse Lines -- Cryopreservation of Transgenic Mouse Lines -- Ovarian Tissue Transplantation and Cryopreservation -- Transgenesis in the Rat -- Transgenesis in the Rat -- Transgenesis in Domestic Species -- Generation of Transgenic Livestock by Pronuclear Injection -- Transgenic Sheep from Cultured Cells -- Characterization and Analysis of Transgenic Strains -- Analysis of Transgenic Mice.
520 _aThe past decade has witnessed a spectacular explosion in both the development and the use of transgenic technologies. In Transgenesis Techniques: Principles and Protocols, Second Edition, authoritative researchers-including members of the Dolly team-take stock of these exciting developments to present a collection of readily reproducible techniques for the generation and analysis of transgenic animals, focusing on the manipulation of the mammalian genome. This second edition devotes much space to the creation of genetically modified murine strains, providing access to the germline both by conventional pronuclear injection and by retroviral and adenoviral infection. Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and conditional (Cre-lox or the Flp-frt systems) gene targeting. Additional chapters provide cutting-edge techniques for the cryopreservation of both male and female germlines, for the generation of transgenic sheep by nuclear transfer, and for the basic analysis of transgenic organisms. Comprehensive and highly practical, this second edition of Transgenesis Techniques: Principles and Protocols updates and expands the acclaimed first edition with a wealth of easy-to-follow cutting-edge protocols and practical tips for producing transgenic animals and analyzing their integrated transgenes.
650 0 _aCytology.
650 1 4 _aCell Biology.
700 1 _aClarke, Alan R.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896036963
776 0 8 _iPrinted edition:
_z9781489939005
776 0 8 _iPrinted edition:
_z9781489939012
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v180
856 4 0 _uhttps://doi.org/10.1385/1592591787
912 _aZDB-2-PRO
999 _c5377
_d5377