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003 DE-He213
005 20260610083627.0
007 cr nn 008mamaa
008 100301s2002 xxu| s |||| 0|eng d
020 _a9781592591886
_9978-1-59259-188-6
024 7 _a10.1385/1592591884
_2doi
050 4 _aRC321-580
072 7 _aPSAN
_2bicssc
072 7 _aSCI089000
_2bisacsh
072 7 _aPSAN
_2thema
082 0 4 _a612.8
_223
245 1 0 _aApoptosis Techniques and Protocols
_h[electronic resource] /
_cedited by Andréa C. LeBlanc.
250 _a2nd ed. 2002.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2002.
300 _aXV, 250 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aNeuromethods,
_x1940-6045 ;
_v37
505 0 _aMolecular Characterization of the Proapoptotic Protein Bax -- Assessing Cytochrome-c Release from Mitochondria -- Monitoring Caspases in Neuronal Cell Death -- HSV Amplicon Vectors in Neuronal Apoptosis Studies -- Microinjections to Study the Specific Role of Proapoptotic Proteins in Neurons -- Designing Microarray Experiments for Neurobiology -- Analysis of Gene Expression by Nylon Membrane cDNA Arrays -- Glyceraldehyde-3-phosphate Dehydrogenase as a Target for Antiapoptotic Drugs -- Analytical Approaches for Investigating Apoptosis and Other Biochemical Events in Compartmented Cultures of Sympathetic Neurons -- Detection and Analysis of Synaptosis -- In Situ Detection of Apoptotic Neurons -- Apoptotic and Oxidative Indicators in Alzheimer’s Disease.
520 _aThe study of the apoptotic process has grown exponentially since the publication of the first edition of Apoptosis Techniques and Protocols for the Neuromethods series in 1997. In this greatly updated second edition, seasoned experts describe in step-by-step detail their best state-of-the-art techniques for studying neuronal cell death. These readily reproducible methods solve a wide variety of research problems, including the detection of the key proteins involved in neuronal apoptosis (Bax protein, cytochrome-c, and caspases), the direct assessment of the role of pro-apoptotic proteins in neurons by viral infections and microinjections, and the detection of proapoptotic proteins in situ. There are also hands-on methods for the study of apoptosis mechanisms in neuronal compartments, for studying synaptosis, and for establishing gene expression profiles in neurodegenerative brain tissues by using DNA microarrays. Written by investigators who have used the techniques extensively, each protocol includes tips on avoiding pitfalls, notes on the method's advantages and disadvantages, and a critical survey of the literature. Cutting-edge and highly practical, Apoptosis Techniques and Protocols, Second Edition, offers both novice and seasoned investigators a rich panoply of the productive tools they need to unravel the molecular mechanisms of neuronal cell death.
650 0 _aNeurosciences.
650 1 4 _aNeuroscience.
700 1 _aLeBlanc, Andréa C.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781588290120
776 0 8 _iPrinted edition:
_z9781617373060
776 0 8 _iPrinted edition:
_z9781489938855
830 0 _aNeuromethods,
_x1940-6045 ;
_v37
856 4 0 _uhttps://doi.org/10.1385/1592591884
912 _aZDB-2-PRO
999 _c5375
_d5375