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_9978-1-0716-1971-1
024 7 _a10.1007/978-1-0716-1971-1
_2doi
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_2bicssc
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082 0 4 _a579.3
_223
245 1 0 _aBacterial Virulence
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Ohad Gal-Mor.
250 _a1st ed. 2022.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2022.
300 _aXII, 267 p. 71 illus., 53 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2427
505 0 _aGeneration of Markerless Gene Deletion Mutants in Listeria monocytogenes Using a Mutated PheS for Counterselection -- A Rapid Fluorescence-Based Screen to Identify Regulators and Components of Interbacterial Competition Mechanisms in Bacteria -- Predicting Type III Effector Proteins Using the Effectidor Web-Server -- Assay for Type III Secretion in Escherichia coli -- Profiling of Secreted Type 3 Secretion System Substrates by Salmonella enterica -- Analysis of SPI-1 Dependent Type III Secretion and Injection Using a NanoLuc Luciferase-Based Assay -- Mycobacterium tuberculosis Infection of THP-1 Cells: A Model for High Content Analysis of Intracellular Growth and Drug Susceptibility -- Bone Marrow-Derived Macrophage (BMDM) Infection by Listeria monocytogenes -- Preparation and Inflammasome Activation of Murine Bone Marrow-Derived and Resident Peritoneal Macrophages -- Flow Cytometry-Based Single Cell Analyses of Bacterial Adaption to Intracellular Environments -- Quantification of Microbial Fluorescent Sensors during Live Intracellular Infections -- Dissecting Human Blood Immune Cells Response to Intracellular Infection Using Single Cell RNA-Sequencing -- Salmonella enterica Infection of Human and Mouse Colon Organoid-Derived Monolayers -- Analysis of Salmonella enterica Adhesion to Leaves of Corn Salad or Lettuce -- Methods for Using the Galleria mellonella Invertebrate Model to Probe Enterococcus faecalis Pathogenicity -- Murine Soft Tissue Infection Model to Study Group A Streptococcus (GAS) Pathogenesis in Necrotizing Fasciitis -- Mouse Model to Study Salmonella-Induced Colitis -- Analysis of Salmonella Typhi Pathogenesis in a Humanized Mouse Model -- In Vivo Tracking of Bacterial Colonization in Different Murine Models Using Bioluminescence: The Example of Salmonella -- Two In Vivo Models to Study Salmonella Asymptomatic Carrier State in Chicks.
520 _aThis detailed volume explores the field of bacterial virulence and the effort to understand how microbial interaction with a host results in the pathology of a specific disease. This collection of selected protocols includes advanced molecular biology and bioinformatics methods, cell culture and organoid models of infection, as well as in vivo infection models that are useful to study the interaction of pathogens with plants, insects, avian, and mammalian hosts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and beneficial, Bacterial Virulence: Methods and Protocols serves as an ideal guide for researchers seeking to promote and further develop the exciting and continuously evolving field of bacterial virulence. Chapter 19 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
650 0 _aBacteria.
650 0 _aMicrobiology.
650 0 _aDiseases
_xCauses and theories of causation.
650 0 _aCytology.
650 0 _aDiseases.
650 1 4 _aBacteria.
650 2 4 _aMicrobiology.
650 2 4 _aPathogenesis.
650 2 4 _aMechanisms of Disease.
700 1 _aGal-Mor, Ohad.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071619704
776 0 8 _iPrinted edition:
_z9781071619728
776 0 8 _iPrinted edition:
_z9781071619735
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2427
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-1971-1
912 _aZDB-2-PRO
999 _c5363
_d5363