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020 _a9781071601914
_9978-1-0716-0191-4
024 7 _a10.1007/978-1-0716-0191-4
_2doi
050 4 _aTP248.13-248.65
072 7 _aTCB
_2bicssc
072 7 _aSCI010000
_2bisacsh
072 7 _aTCB
_2thema
082 0 4 _a660.6
_223
245 1 0 _aAnimal Cell Biotechnology
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Ralf Pörtner.
250 _a4th ed. 2020.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2020.
300 _aXIV, 399 p. 127 illus., 80 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2095
505 0 _aNear-Physiological Cell-Cycle Synchronization with Countercurrent Centrifugal Elutriation -- Turning Cells into Reagents: The Preparation of Assay Ready Cells for Routine Use in Bioassays -- Screening of Media Supplements for High-Performance Perfusion Cultures by Design of Experiment -- ambr® 15 Microbioreactors for CHO Cell Cultivation -- Using a Parallel Micro-Cultivation System (Micro-Matrix) as a Process Development Tool for Cell Culture Applications -- HEK293 Cell-Based Bioprocess Development at Bench Scale by Means of On-Line Monitoring in Shake Flasks (RAMOS and SFR) -- Orbitally Shaken Single-Use Bioreactor for Animal Cell Cultivation: Fed-Batch and Perfusion Mode -- Development of Mammalian Cell Perfusion Cultures at Lab Scale: From Orbitally Shaken Tubes to Benchtop Bioreactors -- Perfusion Control for High Cell Density Cultivation and Viral Vaccine Production -- How to Produce mAbs in a Cube-Shaped Stirred Single-Use Bioreactor at 200 L Scale -- Generic Workflow for the Setup of Mechanistic Process Models -- Estimation of Process Model Parameters -- Efficient Optimization of Process Strategies with Model-Assisted Design of Experiments -- Design, Optimization, and Adaptive Control of Cell Culture Seed Trains -- High-Throughput Quantification and Glycosylation Analysis of Antibodies using Bead-Based Assays -- Surface Plasmon Resonance-Based Method for Rapid Product Sialylation Assessment in Cell Culture -- Analysis of Product Quality of Complex Polymeric IgM Produced by CHO Cells -- Raman Trapping Microscopy for Non-Invasive Analysis of Biological Samples -- An Optical Biosensor for Continuous Glucose Monitoring in Animal Cell Cultures -- Turbidimetry and Dielectric Spectroscopy as Process Analytical Technologies for Mammalian and Insect Cell Cultures -- Continuous Chromatography Purification of Virus-Based Biopharmaceuticals: A Shortcut Design Method -- Single Step Purification of Glycogen Synthase Kinase Isoforms from Small Scale Transient Expression in HEK293 Cells with a Calcium-Dependent Fragment Complementation System.
520 _aAnimal Cell Biotechnology: Methods and Protocols, Fourth Edition constitutes a comprehensive manual of state-of-the-art techniques for setting up mammalian cell lines and media for development of biopharmaceuticals, and optimizing critical parameters for cell culture considering the whole cascade from the lab to the final production. Special emphasis was put on model-assisted concepts. Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in cell line and media development, optimizing process techniques and process strategies, use of model-assisted tools for process design and optimization, and in analysis. Topics include cell line and media development, techniques for process development, model-based techniques for process development, process analysis, and downstream techniques. The volume is divided into five parts that reflect the processes required for different stages of production. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Animal Cell Biotechnology: Methods and Protocols, Fourth Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.
650 0 _aBiotechnology.
650 0 _aPharmaceutical chemistry.
650 1 4 _aBiotechnology.
650 2 4 _aPharmaceutics.
700 1 _aPörtner, Ralf.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071601907
776 0 8 _iPrinted edition:
_z9781071601921
776 0 8 _iPrinted edition:
_z9781071601938
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2095
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-0191-4
912 _aZDB-2-PRO
999 _c5341
_d5341