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020 _a9781493994885
_9978-1-4939-9488-5
024 7 _a10.1007/978-1-4939-9488-5
_2doi
050 4 _aQH324.2-324.25
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082 0 4 _a570.285
_223
082 0 4 _a570.113
_223
245 1 0 _aMetabolomics
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Sanjoy K. Bhattacharya.
250 _a1st ed. 2019.
264 1 _aNew York, NY :
_bSpringer New York :
_bImprint: Humana,
_c2019.
300 _aXV, 335 p. 82 illus., 53 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1996
505 0 _aCollection of Untargeted Metabolomic Data for Mammalian Urine Applying HILIC and Reversed Phase Ultra Performance Liquid Chromatography Methods Coupled to a Q Exactive Mass Spectrometer -- Isotopic Ratio Outlier Analysis (IROA) for Quantitative Analysis -- Nuclear Magnetic Resonance Measurement of Metabolic Flux Using 13C and 1H Signals -- Quantitative Metabolomics using Isotope Residue Outlier Analysis (IROA®) with Internal Standards -- Analyses of Cholesterol Metabolites of Optic Nerve using GC-MS Methods -- Analyses of Cholesterol and Derivatives in Ocular Tissues using LC-MS/MS Methods -- Measurement of Pyridine Nucleotides in Biological Samples using LC-MS/MS -- Handling and Analysis of 5-Formyl-, 5,10-Methenyl-, 10-Formyl-, 5-Formimno-, and 5,10-Methylenetetrahydrofolates -- Protein-Lipid Complex Separation Utilizing a Capillary Electrophoresis System -- Immature and Mature Collagen Crosslinks Quantification using High Performance Liquid Chromatography and High Resolution Mass Spectrometry in OrbitrapTM -- Relative Quantitation of Neurotoxic Metabolites of the Kynurenine Pathway by Laser Desorption Ionization Mass Spectrometry (LDI-MS) -- Analysis of Microbial Siderophores by Mass Spectrometry -- HSD18B7 Enzyme Assay Technique using a Triple Quadrupole Mass Spectrometer -- Assays for Intracellular Cyclic Adenosine Monophosphate (cAMP) and Lysosomal Acidification -- Isotopic Ratio Outlier Analysis (IROA) of Aqueous Humor for Metabolites -- Assessment of Transport of Lipid Metabolites within Trabecular Meshwork Cells -- Detection and Quantification of Lipid Droplets in Differentiated Human Podocytes -- Labeling Cancer Stem Cells with 13C6 Glucose and 13C5 Glutamine for Metabolic Flux Analysis -- Protocols for Metabolomic Analyses from Tissues in Parkinson’s Disease -- Application of Stable Isotope Labels for Metabolomics in Studies in Fatty Liver Disease -- Proteometabolomics ofMelphalan Resistance in Multiple Myeloma -- Metabolomic Analyses of Muscle Atrophy Induced by Hindlimb Unloading -- Protocols for NMR Analysis in Livestock Metabolomics -- Metabolomics Applicable to Retinal Vascular Diseases.
520 _aThe volume brings together some of the best experts in the field of modern metabolomics to discuss various techniques used today to study specific metabolite classes, and metabolomics in bacterial systems and mammalian systems. The chapters in this book cover topics such as Isotopic Ratio Outlier Analysis (IROA) for quantitative analysis; cholesterol and derivatives in ocular tissues using LC-MS/MS methods; microbial siderophores analysis by mass spectrometry; the metabolomic study of tissues in Parkinson’s Disease; and NMR analysis in livestock metabolomics. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, tips on troubleshooting and avoiding pitfalls. Cutting-edge and thorough, Metabolomics: Methods and Protocols is an essential resource for any researcher interestedin this exciting and evolving field. .
650 0 _aBioinformatics.
650 0 _aMass spectroscopy.
650 1 4 _aComputational and Systems Biology.
650 2 4 _aMass Spectrometry.
700 1 _aBhattacharya, Sanjoy K.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781493994878
776 0 8 _iPrinted edition:
_z9781493994892
776 0 8 _iPrinted edition:
_z9781493994908
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1996
856 4 0 _uhttps://doi.org/10.1007/978-1-4939-9488-5
912 _aZDB-2-PRO
999 _c5319
_d5319