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020 _a9781493994540
_9978-1-4939-9454-0
024 7 _a10.1007/978-1-4939-9454-0
_2doi
050 4 _aQH573-671
072 7 _aPSF
_2bicssc
072 7 _aSCI017000
_2bisacsh
072 7 _aPSF
_2thema
082 0 4 _a571.6
_223
245 1 0 _aMass Cytometry
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Helen M. McGuire, Thomas M. Ashhurst.
250 _a1st ed. 2019.
264 1 _aNew York, NY :
_bSpringer New York :
_bImprint: Humana,
_c2019.
300 _aXII, 338 p. 83 illus., 70 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1989
505 0 _aSetting up Mass Cytometry in a Shared Resource Lab Environment -- Acquisition, Processing, and Quality Control of Mass Cytometry Data -- Visualization of Mass Cytometry Signal Background to Enable Optimal Core Panel Customization and Signal Threshold Gating -- Method for Tagging Antibodies with Metals for Mass Cytometry Experiments -- Scalable Conjugation and Characterization of Immunoglobulins with Stable Mass Isotope Reporters for Single-Cell Mass Cytometry Analysis -- Titration of Mass Cytometry Reagents -- Cell-Surface Barcoding of Live PBMC for Multiplexed Mass Cytometry -- Automated Cell Processing for Mass Cytometry Experiments -- Live Cell Barcoding for Efficient Analysis of Small Samples by Mass Cytometry -- Staining of Phosphorylated Signalling Markers Protocol for Mass Cytometry -- Multiplex MHC Class I Tetramer Combined with Intranuclear Staining by Mass Cytometry -- Analysis of the Murine Bone Marrow Haematopoietic System Using Mass and Flow Cytometry -- Mass Cytometric Cell Cycle Analysis -- Picturing Polarized Myeloid Phagocytes and Regulatory Cells by Mass Cytometry -- Quantitative Measurement of Cell-nanoparticle Interactions Using Mass Cytometry -- Data-Driven Flow Cytometry Analysis -- Analysis of Mass Cytometry Data -- Analysis of High Dimensional Phenotype Data Generated by Mass Cytometry or High Dimensional Flow Cytometry -- Computational Analysis of High Dimensional Mass Cytometry Data from Clinical Tissue Samples -- Supervised Machine Learning with CITRUS for Single Cell Biomarker Discovery.
520 _aThis book details a compilation of up-to-date and cutting-edge protocols in mass cytometry.. Chapters guide readers through setting up a facility, panel design and reagent preparation, sample preparation, specific applications, and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mass Cytometry: Methods and Protocols aims to ensure successful results in the further study of this vital field.
650 0 _aCytology.
650 0 _aMass spectroscopy.
650 0 _aImmunospecificity.
650 1 4 _aCell Biology.
650 2 4 _aMass Spectrometry.
650 2 4 _aAdaptive Immunity.
700 1 _aMcGuire, Helen M.
_eeditor.
_0(orcid)0000-0003-2047-6543
_1https://orcid.org/0000-0003-2047-6543
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aAshhurst, Thomas M.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781493994533
776 0 8 _iPrinted edition:
_z9781493994557
776 0 8 _iPrinted edition:
_z9781493994564
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1989
856 4 0 _uhttps://doi.org/10.1007/978-1-4939-9454-0
912 _aZDB-2-PRO
999 _c5316
_d5316