000 04791nam a22005295i 4500
001 978-1-60761-705-1
003 DE-He213
005 20260610083626.0
007 cr nn 008mamaa
008 100803s2010 xxu| s |||| 0|eng d
020 _a9781607617051
_9978-1-60761-705-1
024 7 _a10.1007/978-1-60761-705-1
_2doi
050 4 _aRC666-701.2
072 7 _aMJD
_2bicssc
072 7 _aMED010000
_2bisacsh
072 7 _aMJD
_2thema
082 0 4 _a616.12
_223
245 1 0 _aStem Cells for Myocardial Regeneration
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Randall J. Lee.
250 _a1st ed. 2010.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2010.
300 _aXI, 267 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v660
505 0 _aStem Cells for Myocardial Repair and Regeneration: Where Are We Today? -- Stem Cells for Myocardial Repair and Regeneration: Where Are We Today? -- Isolation and Characterization of Cells for Myocardial Regeneration -- Bone Marrow-Derived Endothelial Progenitor Cells: Isolation and Characterization for Myocardial Repair -- Umbilical Cord Blood Stem Cells for Myocardial Repair and Regeneration -- Isolation of Resident Cardiac Progenitor Cells by Hoechst 33342 Staining -- Mesenchymal Stem Cell Therapy for Cardiac Repair -- Methods for Human Embryonic Stem Cells Derived Cardiomyocytes Cultivation, Genetic Manipulation, and Transplantation -- Animal Models for Assessing Stem Cells for Myocardial Regeneration -- Experimental Cell Transplantation Therapy in Rat Myocardial Infarction Model Including Nude Rat Preparation -- Large Animal Model of Heart Failure for Assessment of Stem Cells -- Histological Assessment of Myocardial Regeneration -- Histopathologic Assessment of Myocardial Regeneration -- Assessment of Myocardial Angiogenesis and Vascularity in Small Animal Models -- Measures of Cell Trafficking -- Superparamagnetic Iron Oxide Labeling of Stem Cells for MRI Tracking and Delivery in Cardiovascular Disease -- Embryonic Stem Cell Biology: Insights from Molecular Imaging -- Genetic Fate-Mapping for Studying Adult Cardiomyocyte Replenishment After Myocardial Injury -- Electrophysiology -- In Vitro Electrophysiological Mapping of Stem Cells -- Assessment of Cardiac Conduction: Basic Principles of Optical Mapping -- Biomaterials -- Surface Patterning for Generating Defined Nanoscale Matrices.
520 _aThere is currently a great deal of enthusiasm surrounding organ regeneration, which mainly comes from the potential pleuripotency of stem cells to differentiate into various tissue types. In Stem Cells for Myocardial Regeneration: Methods and Protocols, expert researchers explore exciting new developments in the field of regenerative medicine, focusing both on the benefits of using stem cells in myocardial repair and regeneration and on the challenges associated with making this a mainstream therapy. Chapters consider the optimal cell type for myocardial repair, examine the developmental processes of the human heart in order to develop strategies for regeneration, consider ex-vivo optical mapping and in vivo electrophysiology studies, and investigate the effects of the extracellular matrix on stem cell renewal and differentiation. Composed in the highly successful Methods in Molecular Biology(tm) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Ground-breaking and current, Stem Cells for Myocardial Regeneration: Methods and Protocols, is an essential guide for scientists, translational investigators, and cardiologists alike, providing readers with a means to evaluate the efficacy and safety of adopting stem cells as the standard for myocardial regeneration.
650 0 _aCardiology.
650 0 _aCytology.
650 0 _aStem cells.
650 0 _aMedical genetics.
650 1 4 _aCardiology.
650 2 4 _aCell Biology.
650 2 4 _aStem Cell Biology.
650 2 4 _aClinical Genetics.
700 1 _aLee, Randall J.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781607617044
776 0 8 _iPrinted edition:
_z9781607617143
776 0 8 _iPrinted edition:
_z9781493961269
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v660
856 4 0 _uhttps://doi.org/10.1007/978-1-60761-705-1
912 _aZDB-2-PRO
999 _c5283
_d5283