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020 _a9781071627242
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024 7 _a10.1007/978-1-0716-2724-2
_2doi
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082 0 4 _a572.865
_223
245 1 0 _aEpigenomics
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Izuho Hatada, Takuro Horii.
250 _a1st ed. 2023.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2023.
300 _aXI, 296 p. 75 illus., 64 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2577
505 0 _aDNA Methylation Analysis Using Bisulfite Pyrosequencing -- Post-Bisulfite Adaptor Tagging Based on an ssDNA Ligation Technique (tPBAT) -- Reduced Representation Bisulfite Sequencing (RRBS) -- Chromatin Immunoprecipitation Sequencing (ChIP-seq) for Detecting Histone Modifications and Modifiers -- ATAC-Seq Analysis of Accessible Chromatin: From Experimental Steps to Data Analysis -- Low-Input CUT&RUN for Mouse Oocytes and Preimplantation Embryos -- Imaging Chromatin Accessibility by Assay of Transposase-Accessible Chromatin with Visualization -- STREAMING-Tag System: Technology to Enable Visualization of Transcriptional Activity and Subnuclear Localization of Specific Endogenous Genes -- Bioinformatics Pipelines for Identification of Super-Enhancers and 3D Chromatin Contacts -- A Method for Detection of Somatic LINE-1 Insertions at the Single Cell Level from Postmortem Human Brain -- Solubilization of Mouse Sperm Chromatin for Sequencing Analyses Using a Chaperon Protein -- Efficient Targeted DNA Methylation with dCas9-Coupled DNMT3A-DNMT3L Methyltransferase -- Regulation of Gene Expression Using dCas9-SunTag Platforms -- Concatenated Coiled-Coil Tag for Highly Efficient, Small Molecule-Inducible Upregulation of Endogenous Mammalian Genes -- Design, Construction, and Validation of Targeted Gene Activation with TREE System in Human Cells -- A Split CRISPR–Cpf1 Platform for Inducible Gene Activation -- Targeted DNA Methylation in Mouse Early Embryos -- Generation of Epigenetic Disease Model Mice by Targeted Demethylation of the Epigenome -- In Vivo Tissue-Specific DNA Demethylation in Mouse Liver through a Hydrodynamic Tail Vein Injection -- Targeted Manipulation of Histone Modification in Medaka Embryos.
520 _aThis detailed volume provides a comprehensive collection of protocols for epigenomic research, powering our ability to analyze epigenetic modifications across the entire genome. Beginning with methods used to investigate epigenomic modifications such as DNA methylation, histone modifications, and chromatin structure, the book continues with methods for manipulating the epigenome, including platforms for epigenome editing, inducible systems for epigenome editing, and epigenetically modified animals. Written for the highly successful Methods in Molecular Biology series, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Epigenomics: Methods and Protocols serves as an ideal resource for researchers looking to further expand the utility and scope of epigenomics research.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aEpigenetics.
650 0 _aBiology
_xTechnique.
650 0 _aGenomics.
650 1 4 _aEpigenetics.
650 2 4 _aGenomic Analysis.
650 2 4 _aGenomics.
700 1 _aHatada, Izuho.
_eeditor.
_0(orcid)0000-0001-7225-3929
_1https://orcid.org/0000-0001-7225-3929
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aHorii, Takuro.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071627235
776 0 8 _iPrinted edition:
_z9781071627259
776 0 8 _iPrinted edition:
_z9781071627266
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2577
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-2724-2
912 _aZDB-2-PRO
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