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020 _a9781071606919
_9978-1-0716-0691-9
024 7 _a10.1007/978-1-0716-0691-9
_2doi
050 4 _aRC321-580
072 7 _aPSAN
_2bicssc
072 7 _aSCI089000
_2bisacsh
072 7 _aPSAN
_2thema
082 0 4 _a612.8
_223
245 1 0 _aVolume Microscopy
_h[electronic resource] :
_bMultiscale Imaging with Photons, Electrons, and Ions /
_cedited by Irene Wacker, Eric Hummel, Steffen Burgold, Rasmus Schröder.
250 _a1st ed. 2020.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2020.
300 _aXVII, 306 p. 107 illus., 88 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aNeuromethods,
_x1940-6045 ;
_v155
505 0 _aCorrelative Super Resolution and Electron Microscopy to Detect Molecules in their Native Cellular Context -- Multi-Color Super-Resolution Microscopy: Revealing the Nano-World of Astrocytes In Situ -- High Resolution Molecular Imaging and Its Applications in Brain and Synapses -- Advancing Array Tomography to Study the Fine Ultrastructure of Identified Neurons in Zebrafish (Danio rerio) -- A Low-Tech Approach to Serial Section Arrays -- Large Volumes in Ultrastructural Neuropathology Imaged by Array Tomography of Routine Diagnostic Samples -- Correlative Ultrastructural Analysis of Functionally Modulated Synapses using Automated Tape-Collecting Ultramicrotome and SEM Array Tomography -- Large-Scale Automated Serial Section Imaging using a Multi-Beam Scanning Electron Microscope -- Improving Serial Blockface SEM by Focal Charge Compensation -- Using X-Ray Microscopy to Increase Targeting Accuracy in Serial Block-Face Scanning Electron Microscopy -- FIBSEM Analysis of Interfaces between Hard Technical Devices and Soft Neuronal Tissue -- Transforming FIB-SEM Systems for Large Volume Connectomics and Cell Biology -- Image Processing for Volume Electron Microscopy -- Forget about Electron Micrographs: A Novel Guide for using 3D Models for Quantitative Analysis of Dense Reconstructions.
520 _aThis volume discusses different approaches to workflows for large volume electron microscopy - from preparation of samples to their imaging in a variety of microscopes - in some cases also applying correlative techniques. The chapters in this book cover topics such as correlative super resolution and electron microscopy to detect molecules in their native cellular context; low-threshold access to serial section arrays; improving serial blockface SEM by focal charge compensation; FIBSEM analysis of interfaces between hard technical devices and soft neuronal tissue; and image processing for volume electron microscopy. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and authoritative, Volume Microscopy: Multiscale Imaging with Photons, Electrons, and Ions is a valuable resource for novice and expert scientists interested in learning more about this evolving field.
650 0 _aNeurosciences.
650 0 _aAnalytical chemistry.
650 0 _aMaterials science.
650 1 4 _aNeuroscience.
650 2 4 _aAnalytical Chemistry.
650 2 4 _aMaterials Science.
700 1 _aWacker, Irene.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aHummel, Eric.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aBurgold, Steffen.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aSchröder, Rasmus.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071606902
776 0 8 _iPrinted edition:
_z9781071606926
776 0 8 _iPrinted edition:
_z9781071606933
830 0 _aNeuromethods,
_x1940-6045 ;
_v155
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-0691-9
912 _aZDB-2-PRO
999 _c5244
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