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020 _a9781493996582
_9978-1-4939-9658-2
024 7 _a10.1007/978-1-4939-9658-2
_2doi
050 4 _aRC321-580
072 7 _aPSAN
_2bicssc
072 7 _aSCI089000
_2bisacsh
072 7 _aPSAN
_2thema
082 0 4 _a612.8
_223
245 1 0 _aMicroglia
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Olga Garaschuk, Alexei Verkhratsky.
250 _a1st ed. 2019.
264 1 _aNew York, NY :
_bSpringer New York :
_bImprint: Humana,
_c2019.
300 _aXII, 340 p. 65 illus., 6 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2034
505 0 _aMicroglia: The Neural Cells of Non-Neural Origin -- Morphology of Microglia across Contexts of Health and Disease -- Physiology of Microglia -- Microglia Reactivity: Heterogeneous Pathological Phenotypes -- Microglia in Neurodegenerative Disorders -- Physiological Implications of Microglia-Synapse Interactions -- Ultrastructural Analyses of Microglial Interactions with Synapses -- Visualizing Dark Microglia -- Electrophysiological Investigation of Microglia -- Replenishment of Organotypic Hippocampal Slice Cultures with Neonatal or Adult Microglia -- Assessment of Parasite-Microglia Interactions In Vitro -- In Vivo Visualization of Microglia Using Tomato Lectin -- Long-Term In Vivo Imaging of Individual Microglial Cells -- Analysis of Signaling Mechanisms Regulating Microglial Process Movement -- Measuring Microglial Turnover in the Adult Brain -- Novel Microglia Depletion Systems: A Genetic Approach Utilizing Conditional Diphtheria Toxin Receptor Expression and a Pharmacological Model Based on the Blocking of Macrophage Colony-Stimulating Factor 1 Receptor -- Single Cell Electroporation for Measuring In Vivo Calcium Dynamics in Microglia -- Labeling Microglia with Genetically-Encoded Calcium Indicators -- Calcium Imaging of Microglial Network Activity in Stroke -- In Vivo Two Photon Imaging of Microglial Synapse Contacts -- In Vivo Phagocytosis Analysis of Amyloid Beta -- Functional Analysis of Brain-Engrafted Monocytes after Microglial Ablation in Mouse Models -- Analysis of the Microglial Sensome -- Transcriptomics and Live Imaging to Define Functional Phenotypes of Microglia in Pathological Human Tissue.
520 _aThis book presents a comprehensive toolkit of versatile techniques for studying microglia under different experimental settings along with a brief summary of knowledge, accumulated in microglial research over the last decades. Beginning with recently discovered roles of microglia in health and disease, the volume continues by covering in vitro analyses of microglia, in vivo studies, and “omics” analyses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Microglia: Methods and Protocols serves as a vital guide to these important cells and an inspiration for scientists interested in expanding our knowledge of their role in the nervous system.
650 0 _aNeurosciences.
650 0 _aImmunology.
650 1 4 _aNeuroscience.
650 2 4 _aImmunology.
700 1 _aGaraschuk, Olga.
_eeditor.
_0(orcid)0000-0001-7400-5654
_1https://orcid.org/0000-0001-7400-5654
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_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aVerkhratsky, Alexei.
_eeditor.
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_1https://orcid.org/0000-0003-2592-9898
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781493996575
776 0 8 _iPrinted edition:
_z9781493996599
776 0 8 _iPrinted edition:
_z9781493996605
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2034
856 4 0 _uhttps://doi.org/10.1007/978-1-4939-9658-2
912 _aZDB-2-PRO
999 _c5219
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