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020 _a9781493987399
_9978-1-4939-8739-9
024 7 _a10.1007/978-1-4939-8739-9
_2doi
050 4 _aRC321-580
072 7 _aPSAN
_2bicssc
072 7 _aSCI089000
_2bisacsh
072 7 _aPSAN
_2thema
082 0 4 _a612.8
_223
245 1 0 _aSynaptosomes
_h[electronic resource] /
_cedited by Kathryn M. Murphy.
250 _a1st ed. 2018.
264 1 _aNew York, NY :
_bSpringer New York :
_bImprint: Humana,
_c2018.
300 _aXV, 312 p. 55 illus., 32 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aNeuromethods,
_x1940-6045 ;
_v141
505 0 _aIntroduction to Synaptosome Methods and Applications -- Victor P. Whittaker: The Discovery of the Synaptosome and Its Implications -- Synaptosome Preparations: Which Procedure Should I Use? -- Protocol for a High-Throughput Semi-Automated Preparation for Filtered Synaptoneurosomes -- Subcellular Fractionation of Brain Tissue from Small Tissue Explants -- Preparation of P2 of Percoll-Purified Synaptosomes from Mammalian Brain Tissue -- Synaptosomal Bioenergetics and Glutamate Release -- Synaptosomes Bioenergetics and Calcium Handling: Aging Response -- Isolation of Highly Purified and Functionally Active Synaptic Mitochondria -- Affinity Proteomics for Interactome and Phosphoproteome Screening in Synaptosomes -- Manipulating Synapse-Enriched Biochemical Fractions to Detect a Purified Synaptic Protein’s Effect on Neurotransmitter Release -- Prosomes: Supramolecular Structures at the Synaptosome Membrane Involved in Vesicle Docking, Fusion,and Neurotransmitter Release -- SMPL-Synaptic Membranes: Nanodisc-Mediated Synaptic Membrane Mimetics Expand the Toolkit for Drug Discovery and the Molecular Cell Biology of Synapses -- Profiling of MicroRNA and Protein from Purified Synaptoneurosomes in a Neurodegenerative Disease Model -- Use of Synaptoneurosome Samples to Study Development and Plasticity of Human Cortex -- The Use of Synaptosomes in Studying Autism Spectrum Disorder and Other Neurodevelopmental Disorders -- Flow Cytometric Evaluation of Crude Synaptosome Preparation as a Way to Study Synaptic Alteration in Neurodegenerative Diseases.
520 _aThis volume describes techniques to make synaptosomes, and discusses numerous ways that synaptosomes are used to study and answer diverse questions about synapses. After examining the history that led to making synaptosomes, the chapters delved into its important as a tool to explore synaptic connections. Some of the topics covered in this book are: synaptosome bioenergetics and glutamate release; affinity proteomics for interactome and phosphoproteome screening in synaptosomes; isolation of highly purified and functionally active synaptic mitochondria; and profiling of microRNA and protein from purified synaptoneurosomes in a neurodegenerative disease model. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and thorough, Synaptosomes is a valuable resource for any researcher interested in learning more about this developing field.
650 0 _aNeurosciences.
650 1 4 _aNeuroscience.
700 1 _aMurphy, Kathryn M.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781493987382
776 0 8 _iPrinted edition:
_z9781493987405
776 0 8 _iPrinted edition:
_z9781493993796
830 0 _aNeuromethods,
_x1940-6045 ;
_v141
856 4 0 _uhttps://doi.org/10.1007/978-1-4939-8739-9
912 _aZDB-2-PRO
999 _c5193
_d5193