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020 _a9781071629703
_9978-1-0716-2970-3
024 7 _a10.1007/978-1-0716-2970-3
_2doi
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245 1 0 _aDrosophila Oogenesis
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Michelle S. Giedt, Tina L. Tootle.
250 _a1st ed. 2023.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2023.
300 _aXIII, 447 p. 92 illus., 82 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2626
505 0 _aThe Vast Utility of Drosophila Oogenesis -- Visualization and Quantification of Drosophila Larval Ovaries -- Dissection, Fixation, and Standard Staining of Adult Drosophila Ovaries -- Utilizing the FLP-Out System for Clonal RNAi Analysis in the Adult Drosophila Ovary -- Analysis of Physiological Control of Adult Drosophila Oogenesis by Inter-Organ Communication -- Immunohistochemical Analysis of Nuclear Lamina Structures in the Drosophila Ovary Using CRISPR-Tagged Genes -- Visualizing Fusome Morphology via Tubulin Immunofluorescence in Drosophila Ovarian Germ Cells -- Tracking Follicle Cell Development -- Optimized Fixation and Phalloidin Staining of Basally Localized F-Actin Networks in Collectively Migrating Follicle Cells -- Quantitative Image Analysis of Dynamic Cell Behaviors during Border Cell Migration -- Live Imaging of Nurse Cell Behavior in Late Stages of Drosophila Oogenesis -- Visualizing Lipid Droplets in Drosophila Oogenesis -- Assessing Ovulation in Drosophila melanogaster -- A Low-Tech Flow Chamber for Live Imaging of Drosophila Egg Chambers during Drug Treatments -- Detection and Assessment of Wolbachia pipientis Infection -- Measuring Transposable Element Activity in Adult Drosophila Ovaries -- Preparation of Drosophila Ovarioles for Single-Cell RNA Sequencing -- Chromatin Immunoprecipitation Experiments from Drosophila Ovaries -- Detection of Actin in Nuclear Protein Fraction Isolated from Adult Drosophila Ovary -- STAMP: Spatio-Temporal Association Mapping of Proteins -- Using Drosophila Oogenesis in the Classroom to Increase Student Participation in Biomedical Research -- Gaining Wings to FLY: Using Drosophila Oogenesis as an Entry Point for Citizen Scientists in Laboratory Research.
520 _aThis detailed volume compiles numerous methods to explore fly oogenesis. Beginning with updated protocols from isolating and staining the ovary to numerous imaging techniques and genetic protocols for cell-specific assessment and CRISPR-mediated mutagenesis, the volume continues by detailing techniques from assessing cytoskeletal structures to uncovering protein-protein interactions, with closing chapters exploring how Drosophila oogenesis can be used in the classroom and in outreach programs to increase interest in biomedical research, STEM education, and STEM careers. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Drosophila Oogenesis: Methods and Protocols serves as an ideal guide for both new and expert researchers working with this robust and versatile model organism.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aBiological models.
650 0 _aDevelopmental biology.
650 1 4 _aBiological Models.
650 2 4 _aDevelopmental Biology and Stem Cells.
700 1 _aGiedt, Michelle S.
_eeditor.
_0(orcid)0000-0002-5720-8326
_1https://orcid.org/0000-0002-5720-8326
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aTootle, Tina L.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071629697
776 0 8 _iPrinted edition:
_z9781071629710
776 0 8 _iPrinted edition:
_z9781071629727
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2626
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-2970-3
912 _aZDB-2-PRO
999 _c5160
_d5160