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020 _a9781493996629
_9978-1-4939-9662-9
024 7 _a10.1007/978-1-4939-9662-9
_2doi
050 4 _aRC321-580
072 7 _aPSAN
_2bicssc
072 7 _aSCI089000
_2bisacsh
072 7 _aPSAN
_2thema
082 0 4 _a612.8
_223
245 1 0 _aNeuroproteomics
_h[electronic resource] /
_cedited by Ka Wan Li.
250 _a2nd ed. 2019.
264 1 _aNew York, NY :
_bSpringer New York :
_bImprint: Humana,
_c2019.
300 _aXIII, 218 p. 55 illus., 37 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aNeuromethods,
_x1940-6045 ;
_v146
505 0 _aNeuroproteomics: The Methods -- Dissection of Rodent Brain Regions: Guided Free-Hand Slicing and Dissection of Frozen Tissue -- Isolation of Synapse Sub-Domains by Subcellular Fractionation Using Sucrose Density Gradient Centrifugation: Purification of the Synaptosome, Synaptic Plasma Membrane, Postsynaptic Density, Synaptic Membrane Raft, and Postsynaptic Density Lattice -- Laser Microdissection for Spatial Proteomics of Post Mortem Brain Tissue -- High pH Reversed-Phase Pre-Fractionation for In-Depth Shotgun Proteomics -- Sample Preparation for Proteomics Analysis: Filter-Aided Sample Preparation (FASP) and Single-Pot Solid-Phase Sample Preparation (SP3) -- Enrichment of Low-Molecular-Weight Phosphorylated Biomolecules Using Phos-Tag Tip -- Integrated Immunoprecipitation: Blue Native Gel Electrophoresis - Mass Spectrometry for the Identification of Protein Sub-Complexes -- Analysis of Synaptic Protein-Protein Interaction by Crosslinking-Mass Spectrometry -- TMT-MS3 Enabled Proteomic Quantification of Human IPSC Derived Neurons -- Data Independent Acquisition (SWATH) Mass Spectrometry Analysis of Protein Content in Primary Neuronal Cultures -- Quantitative Analysis of Mass Spectrometry-Based Proteomics Data -- 2D-DIGE as a Tool in Neuroproteomics -- Neuropeptidomics of the Mammalian Brain -- MALDI Imaging Mass Spectrometry - Neurochemical Imaging of Proteins and Peptides -- A Combined Cellomics and Proteomics Approach to Uncover Neuronal Pathways to Psychiatric Disorder.
520 _aThis second edition volume expands on the previous edition with updates on the latest techniques used in the field of proteomics. Tissue and cellular proteins are now being analyzed at near proteome-wide levels and mass spectrometers are achieving higher sensitivity and speed, resolution, and accuracy. The chapters in this book cover various new methods used in proteomic workflows such as laser microdissection for spatial proteomics of post-mortem brain tissue, sample preparation in single vessel with FASP and SP3 protocols, the crosslinking mass spectrometry, TMT-MS3 based proteomic with synchronous precursor selection, mass spectrometric data dependent acquisition (DDA) and data independent acquisition (DIA, SWATH) methods, 2D-DIGE, and MALDI Imaging Mass Spectrometry. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, Neuroproteomics, Second Edition is a valuable guide that help researchers obtain the finest results from current proteomics platforms.
650 0 _aNeurosciences.
650 0 _aProteins .
650 1 4 _aNeuroscience.
650 2 4 _aProtein Biochemistry.
700 1 _aLi, Ka Wan.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781493996612
776 0 8 _iPrinted edition:
_z9781493996636
776 0 8 _iPrinted edition:
_z9781493996643
830 0 _aNeuromethods,
_x1940-6045 ;
_v146
856 4 0 _uhttps://doi.org/10.1007/978-1-4939-9662-9
912 _aZDB-2-PRO
999 _c5130
_d5130