| 000 | 04529nam a22005295i 4500 | ||
|---|---|---|---|
| 001 | 978-1-4939-9558-5 | ||
| 003 | DE-He213 | ||
| 005 | 20260610083623.0 | ||
| 007 | cr nn 008mamaa | ||
| 008 | 190620s2019 xxu| s |||| 0|eng d | ||
| 020 |
_a9781493995585 _9978-1-4939-9558-5 |
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| 024 | 7 |
_a10.1007/978-1-4939-9558-5 _2doi |
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| 050 | 4 | _aSB621-795 | |
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_aPSTP _2bicssc |
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_aPSTB _2thema |
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| 082 | 0 | 4 |
_a571.92 _223 |
| 245 | 1 | 0 |
_aCitrus Tristeza Virus _h[electronic resource] : _bMethods and Protocols / _cedited by Antonino F. Catara, Moshe Bar-Joseph, Grazia Licciardello. |
| 250 | _a1st ed. 2019. | ||
| 264 | 1 |
_aNew York, NY : _bSpringer New York : _bImprint: Humana, _c2019. |
|
| 300 |
_aX, 247 p. 50 illus., 36 illus. in color. _bonline resource. |
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| 336 |
_atext _btxt _2rdacontent |
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| 337 |
_acomputer _bc _2rdamedia |
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| 338 |
_aonline resource _bcr _2rdacarrier |
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| 347 |
_atext file _bPDF _2rda |
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| 490 | 1 |
_aMethods in Molecular Biology, _x1940-6029 ; _v2015 |
|
| 505 | 0 | _aA Short Note on Reflections and Publications on Citrus Tristeza Virus (CTV) Methodologies -- A Brief Historical Account of the Family Closteroviridae -- Phenotyping Biological Properties of CTV Isolates -- CTV Vectors and Interactions with the Virus and Host Plants -- Tissue Print and Squash Captures Real-Time RT-PCR Method for Direct Detection of Citrus Tristeza Virus (CTV) in Plant or Vector Tissues -- Detection of Citrus Tristeza Virus and Co-Infecting Viroids -- Assessment of Genetic Variability of Citrus Tristeza Virus by SSCP and CE-SSCP -- Identification and Characterization of Resistance-Breaking (RB) Isolates of Citrus Tristeza Virus -- Genotyping Citrus Tristeza Virus Isolates by Sequential Multiplex RT-PCR and Microarray Hybridization in a Lab-On-Chip Device -- Rapid and Sensitive Detection of Citrus Tristeza Virus Using Reverse Transcription-Loop-Mediated Isothermal Ampliļ¬cation (RT-LAMP) Assay -- Amplification and Cloning of Large cDNA Fragments of the Citrus Tristeza Virus Genome -- Bioinformatic Tools and Genome Analysis of Citrus Tristeza Virus -- Analysis of Genotype Composition of Citrus Tristeza Virus Populations Using Illumina Miseq Technology -- Citrus Tristeza Virus: Host RNA Silencing and Virus Counteraction -- Proteomic Response of Host Plants to Citrus Tristeza Virus -- Gene Expression in Citrus Plant Cells Using HeliosĀ® Gene Gun System for Particle Bombardment -- Methods for Producing Transgenic Plants Resistant to CTV. | |
| 520 | _aThis book provides methods and clear protocols for the various technologies available to detect, characterize, and study Citrus tristeza virus (CTV), a member of the genus Closterovirus, family Closteroviridae. Thanks to the highly sensitive and specific diagnostic procedures developed, knowledge of the molecular characteristics, expression strategies, genetic variability, and epidemiology of the virus has improved significantly, as this volume reflects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Citrus Tristeza Virus: Methods and Protocols serves as an ideal guide for plant pathologists, plant virologists, molecular biologists, and graduate students interested in performing qualitative and quantitative tests as well as recently-developed diagnostic methods in order to find solutions to improve the management of this disease. | ||
| 650 | 0 | _aPlant diseases. | |
| 650 | 0 | _aVirology. | |
| 650 | 0 | _aPlant genetics. | |
| 650 | 1 | 4 | _aPlant Pathology. |
| 650 | 2 | 4 | _aVirology. |
| 650 | 2 | 4 | _aPlant Genetics. |
| 700 | 1 |
_aCatara, Antonino F. _eeditor. _4edt _4http://id.loc.gov/vocabulary/relators/edt |
|
| 700 | 1 |
_aBar-Joseph, Moshe. _eeditor. _4edt _4http://id.loc.gov/vocabulary/relators/edt |
|
| 700 | 1 |
_aLicciardello, Grazia. _eeditor. _4edt _4http://id.loc.gov/vocabulary/relators/edt |
|
| 710 | 2 | _aSpringerLink (Online service) | |
| 773 | 0 | _tSpringer Nature eBook | |
| 776 | 0 | 8 |
_iPrinted edition: _z9781493995578 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781493995592 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781493995608 |
| 830 | 0 |
_aMethods in Molecular Biology, _x1940-6029 ; _v2015 |
|
| 856 | 4 | 0 | _uhttps://doi.org/10.1007/978-1-4939-9558-5 |
| 912 | _aZDB-2-PRO | ||
| 999 |
_c5125 _d5125 |
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