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020 _a9781493991709
_9978-1-4939-9170-9
024 7 _a10.1007/978-1-4939-9170-9
_2doi
050 4 _aRB155-155.8
072 7 _aMFN
_2bicssc
072 7 _aMED107000
_2bisacsh
072 7 _aMFN
_2thema
082 0 4 _a616.042
_223
245 1 0 _aCRISPR Gene Editing
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Yonglun Luo.
250 _a1st ed. 2019.
264 1 _aNew York, NY :
_bSpringer New York :
_bImprint: Humana,
_c2019.
300 _aXI, 362 p. 76 illus., 61 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1961
505 0 _aCRISPR-gRNA Design -- Tracking CRISPR’s Footprints -- Rapid Quantitative Evaluation of CRISPR Genome Editing by TIDE and TIDER -- Fast and Quantitative Identification of Ex Vivo Precise Genome Targeting-Induced Indel Events by IDAA -- Functional Evaluation of CRISPR Activity by the Dual-Fluorescent Surrogate System: C-Check -- CRISPR-Cas9 Delivery by Artificial Virus (RRPHC) -- Production and Validation of Lentiviral Vectors for CRISPR/Cas9 Delivery -- Rapid and Simple Screening of CRISPR Guide RNAs (gRNAs) in Cultured Cells Using Adeno-Associated Viral (AAV) Vectors -- Electroporation-Based CRISPR/Cas9 Gene Editing Using Cas9 Protein and Chemically Modified sgRNAs -- Efficient Gene Editing of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9 -- Editing the Genome of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9 Ribonucleoprotein Complexes -- Conditional Gene Knockout in Human Cells with Inducible CRISPR/Cas9 -- CRISPR/Cas9 as a Genome Editing Tool for Targeted Gene Integration in CHO Cells -- Rapid and Efficient Gene Deletion by CRISPR/Cas9 -- Genome Editing in Mice -- CRISPR/Cas9-Mediated Gene Tagging: A Step-by-Step Protocol -- Gene Editing in Primary Cells of Cattle and Pig -- Toward In Vivo Gene Therapy Using CRISPR -- CRISPR Gene Therapy of the Eye: Targeted Knockout of Vegfa in Mouse Retina by Lentiviral Delivery -- In Vivo Editing of the Adult Mouse Liver Using CRISPR/Cas9 and Hydrodynamic Tail Vein Injection -- CRISPR-Based Lentiviral Knockout Libraries for Functional Genomic Screening and Identification of Phenotype-Related Genes.
520 _aThis detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPRgene editing field. Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.
650 0 _aMedical genetics.
650 0 _aBiotechnology.
650 1 4 _aMedical Genetics.
650 2 4 _aChemical Bioengineering.
700 1 _aLuo, Yonglun.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781493991693
776 0 8 _iPrinted edition:
_z9781493991716
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1961
856 4 0 _uhttps://doi.org/10.1007/978-1-4939-9170-9
912 _aZDB-2-PRO
999 _c5117
_d5117