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020			_a9781493989911 _9978-1-4939-8991-1
024	7		_a10.1007/978-1-4939-8991-1 _2doi
050		4	_aQK
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245	1	0	_aPlant Genome Editing with CRISPR Systems _h[electronic resource] : _bMethods and Protocols / _cedited by Yiping Qi.
250			_a1st ed. 2019.
264		1	_aNew York, NY : _bSpringer New York : _bImprint: Humana, _c2019.
300			_aXV, 367 p. 73 illus., 65 illus. in color. _bonline resource.
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
347			_atext file _bPDF _2rda
490	1		_aMethods in Molecular Biology, _x1940-6029 ; _v1917
505	0		_aPlant DNA Repair Pathways and their Applications in Genome Editing -- Rapid Screening of CRISPR/Cas9-Induced Mutants using the ACT-PCR Method -- Decoding Sanger-Sequencing Chromatograms from CRISPR-Induced Mutations -- Creating Large Chromosomal Deletions in Rice using CRISPR/Cas9 -- A Multiplexed CRISPR-Cas9 Editing System Based on the Endogenous tRNA Processing -- A Single Transcript CRISPR-Cas9 System for Multiplex Genome Editing in Plants -- CRISPR-Act2.0: An Improved Multiplexed System for Plant Transcriptional Activation -- Generating Photoperiod-Sensitive Genic Male Sterile Rice Lines with CRISPR-Cas9 -- Knocking Out MicroRNA Genes in Rice with CRISPR/Cas9 -- An Agrobacterium-Mediated CRISPR-Cas9 Platform for Genome Editing in Maize -- Fluorescence Marker-Assisted Isolation of Cas9-Free and CRISPR-Edited Arabidopsis Plants -- Creating Targeted Gene Knock-Outs in Brassica oleracea using CRISPR/Cas9 -- Application of CRISPR/Cas9-Mediated Gene Editing in Tomato -- Genome Editing in Potato with CRISPR/Cas9 -- Visual Assay for Gene Editing using a CRISPR/Cas9 System in Carrot Cells -- Genome Editing in Soybean with CRISPR/Cas9 -- Genome Editing in Citrus Tree with CRISPR/Cas9 -- Plant Gene Knockout and Knockdown by CRISPR-Cpf1 (Cas12a) Systems -- Editing a Stomatal Developmental Gene in Rice with CRISPR/Cpf1 -- Targeted Mutagenesis using FnCpf1 in Tobacco -- Gene Replacement by Intron Targeting with CRISPR-Cas9 -- Targeted Base Editing with CRISPR-Deaminase in Tomato -- Virus-Mediated Genome Editing in Plants using the CRISPR/Cas9 System -- Biolistic Delivery of CRISPR/Cas9 with Ribonucleoprotein Complex in Wheat -- DNA-Free Genome Editing via Ribonucleoprotein (RNP) Delivery of CRISPR/Cas in Lettuce -- An Automated Protoplast Transformation System.
520			_aThis volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems; CRISPR-Cas12a (Cpf1) editing systems; and non-agrobacterium based CRISPR delivery systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Plant Genome Editing with CRISPR Systems: Methods and Protocols is a valuable resource for any researcher interested in learning about and using CRISPR systems in plants.
650		0	_aBotany.
650		0	_aPlant genetics.
650	1	4	_aPlant Science.
650	2	4	_aPlant Genetics.
700	1		_aQi, Yiping. _eeditor. _4edt _4http://id.loc.gov/vocabulary/relators/edt
710	2		_aSpringerLink (Online service)
773	0		_tSpringer Nature eBook
776	0	8	_iPrinted edition: _z9781493989904
776	0	8	_iPrinted edition: _z9781493989928
830		0	_aMethods in Molecular Biology, _x1940-6029 ; _v1917
856	4	0	_uhttps://doi.org/10.1007/978-1-4939-8991-1
912			_aZDB-2-PRO
999			_c5109 _d5109